{{get_dataset_fail}}




{{section.text}} {{section.text}} {{section.text}} {{section.text}} {{dataset.name}}


Complete global brain ischemia (CGBI) and reperfusion occur following resuscitation from cardiac arrest. Different brain neurons are selectively vulnerable to CGBI: pyramidal neurons of hippocampal CA3 survive 10 min CGBI but those of CA1 die at 3 days following 10 min CGBI. CA3 neurons are expected to have more robust stress responses and repair responses than CA1 neurons. We used microarrays to compared total and polysome-bound mRNAs in CA1 and CA3 at 8 hr reperfusion after 10 min CGBI in Long Evans male rats to ascertain differences in total vs polysome-bound gene expression. Male Long Evans rats were subjected to (1) sham operation (non-ischemic control, NIC) or normothermic CGBI of 10 min followed by 8 hr reperfusion (8R). Hippocampal CA1 and CA3 were dissected. n = 5 CA1 or CA3 were pooled to give a single replicate and there were 3 or 4 replicates per group. Post-mitochondrial supernatant (PMS) was prepared. Twenty percent of PMS was TRIzol extracted to give total RNA. The remainder was run on a 20% sucrose pad to isolate polysome pellets, which were also TRIzol extracted to give polysome RNA. Total and polysome RNA were then run on Affymetrix Rat Gene 2.0 microarrays.

ABSTRACT: {{section.text}} {{section.text}} {{section.text}} {{section.text}} {{abstract_sections[abstract_sections.length-1].tobeReduced=='true'?"... [more]":""}} [less]

SAMPLE PROTOCOL: {{section.text}} {{section.text}} {{section.text}} {{section.text}} {{sample_protocol_sections[sample_protocol_sections.length-1].tobeReduced=='true'?"... [more]":""}} [less]

DATA PROTOCOL: {{section.text}} {{section.text}} {{section.text}} {{section.text}} {{data_protocol_sections[data_protocol_sections.length-1].tobeReduced=='true'?"... [more]":""}} [less]

REANALYSIS of: {{reanalysis_item.accession}}

REANALYZED by: {{reanalyzed_item.accession}}

OTHER RELATED OMICS DATASETS IN: {{reanalysis_item.accession}}

INSTRUMENT(S): {{instrument+';'}}

ORGANISM(S): {{organism.name + ';'}}

TISSUE(S): {{tissue+';'}}

DISEASE(S): {{disease+';'}}

SUBMITTER: {{dataset['submitter']}}

PROVIDER: {{acc}} | {{repositories[domain]}} | {{dataset['publicationDate']}}

{{publication_info[publication_index_info[dataset.publicationIds[current_publication]]].title}}

{{author.fullname.substr(0,author.fullname.length-2)}} ,

{{publication_info[publication_index_info[dataset.publicationIds[current_publication]]].citation}}


Sorry, this publication's infomation has not been loaded in the Indexer, please go directly to PUBMED or Altmetric.

ABSTRACT: {{publication_info[publication_index_info[dataset.publicationIds[current_publication]]].pub_abstract[0]}}
{{publication_info[publication_index_info[dataset.publicationIds[current_publication]]].pub_abstract[1]}} [less]

ABSTRACT: {{publication_info[publication_index_info[dataset.publicationIds[current_publication]]].pub_abstract[0]|limitTo:500}} {{publication_info[publication_index_info[dataset.publicationIds[current_publication]]].pub_abstract[0].length>500?"... [more]":""}}

Publication: {{current_publication +1}}/{{dataset.publicationIds.length}}

{{dataset.publicationIds[current_publication].publicationDate}}


Only show the datasets with similarity scores above:{{threshold}}

Threshold:
    {{threshold}}
     

The biological similarity score is calculated based on the number of molecules (Proteins, Metabolites, Genes) common between two different projects.

Similar Datasets

  • Organism: {{organism["name"]}} Not available
    {{relatedDataset['publicationDate'].substr(0,4)+"-"+relatedDataset['publicationDate'].substr(4,2)+"-"+relatedDataset['publicationDate'].substr(6,2)}}| {{relatedDataset.id}} | {{repositories[relatedDataset.source]}}