Transcription profiling by array of pneumococcal virulence factors expressed during meningitis in mice

ABSTRACT: Streptococcus pneumoniae is the most common cause of severe bacterial meningitis in children, the elderly, and immunocompromised individuals. To identify virulence factors preferentially expressed during meningitis, we conducted niche-specific genome-wide in vivo transcriptomic analysis after intranasal infection of mice with serotype 4 or 6A pneumococci. The expression of 34 bacterial genes was substantially altered in brain tissue of mice infected with either of the 2 strains. Ten upregulated genes were common to both strains, 7 of which were evaluated for their role in the development of meningitis. One previously uncharacterized protein, alpha-glycerophosphate oxidase (GlpO), was cytotoxic for human brain microvascular endothelial cells (HBMECs) via generation of H2O2. A glpO deletion

ORGANISM(S): Streptococcus pneumoniae

SUBMITTER: Adam Witney 

PROVIDER: E-BUGS-130 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress