Unknown,Transcriptomics,Genomics,Proteomics

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Budding yeast cohesin (Mcd1) distribution in mitotically arrested cells


ABSTRACT: genomic localization of the budding yeast cohesin complex was mapped in mitotically arrested cells by Mcd1 ChIP followed by hybridization to high-density tiled microarrays Cells were synchronized first in G1 and then released into media containing the microtubule poison nocodazole. Cells were fixed and processed for ChIP once arrested as large-budded cells. Immunoprecipitated DNA and total genomic DNA not subjected to immunoprecipitation were competitively hybridized to Nimblegen whole genome arrays.

ORGANISM(S): Saccharomyces cerevisiae

SUBMITTER: Paul Megee 

PROVIDER: E-GEOD-18021 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

The Scc2/Scc4 cohesin loader determines the distribution of cohesin on budding yeast chromosomes.

Kogut Igor I   Wang Jianbin J   Guacci Vincent V   Mistry Rohinton K RK   Megee Paul C PC  

Genes & development 20091001 19


Cohesins mediate sister chromatid cohesion and DNA repair and also function in gene regulation. Chromosomal cohesins are distributed nonrandomly, and their deposition requires the heterodimeric Scc2/Scc4 loader. Whether Scc2/Scc4 establishes nonrandom cohesin distributions on chromosomes is poorly characterized, however. To better understand the spatial regulation of cohesin association, we mapped budding yeast Scc2 and Scc4 chromosomal distributions. We find that Scc2/Scc4 resides at previously  ...[more]

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