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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Transcriptional effects of chronic heroin and methamphetamine treatment in the mouse striatum

ABSTRACT: To identify molecular effects of chronic drug treatment, heroin and methamphetamine treated animals were compared with saline treated animals at multiple time-points using microarray technology. Gene expression profile was assessed 14 h after the last dose of 1, 3, 6 or 12 days drug treatment and after 13, 15, 18 or 24 days of withdrawal. Animals were injected intraperitoneally with saline (SAL) (Polfa, Lublin, Poland), heroin (synthesized from morphine in Institute of Pharmacology PAS, Krakow, Poland) or D-methamphetamine (Sigma-Aldrich, Poznan, Poland) twice a day for consecutive 12 days in increasing doses. The Methamphetamine last dose (8 mg/kg) was four times greater than the first dose (2 mg/kg). It was also the case for heroin (40 and 10 mg/kg respectively). Mice were sacrificed by decapitation after 1, 3, 6 or 12 days of treatment or after 13, 15, 18 or 24 days of withdrawal.

ORGANISM(S): Mus musculus

SUBMITTER: Marcin Piechota

PROVIDER: E-GEOD-30305 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Common transcriptional effects in the mouse striatum following chronic treatment with heroin and methamphetamine.

Piechota M M Korostynski M M Sikora M M Golda S S Dzbek J J Przewlocki R R

Genes, brain, and behavior 20120411 4

The molecular alterations that underlie the long-lasting behavioural effects of drugs of abuse, such as psychomotor sensitization and physical dependence, are still not known. Moreover, it is not known which molecular effects are similar for addictive drugs from various pharmacological classes. In this study, we utilized whole-genome microarray profiling to evaluate the detailed time-course of transcriptional alterations in the mouse striatum during chronic treatment with heroin (HER) and metham ...[more]

PMID: 22390687

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