Regulation of cholesterogenic gene expression in Japanese medaka (Oryzias latipes) exposed to 17M-NM-2-trenbolone

ABSTRACT: The anabolic androgen 17M-NM-2-trenbolone (TB) can cause masculinization and reduce fecundity of fish. However, the underlying mechanisms of various biological pathways including metabolism, biosynthesis etc. are largely unknown. Here, we evaluated the effects of TB using the medaka DNA microarray representing 36,398 genes. Larval medaka, Oryzias latipes, (within 24 hrs posthatch) were exposed to TB at various concentrations (2, 6, 20, 60, 100, 200 ng/L) for up to 7 days. Dose-response relationships in gene expression levels of the categorized genes were analyzed using the cumulative chisquared method. Microarray analyses of the TB-exposed larvae showed that 117 and 32 genes were determined as up and down-regulated genes, respectively. The most significant GO term for biological process id

ORGANISM(S): Oryzias latipes

SUBMITTER: Katsuyuki Kadota 

PROVIDER: E-GEOD-35908 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress