Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Functional characterization of the RNA chaperone Hfq in opportunistic human pathogen Stenotrophomonas maltophilia

ABSTRACT: Stenotrophomonas maltophilia is an important opportunistic pathogen affecting primarily hospitalized and immuno-compromised hosts. We constructed an hfq deletion mutant (Delta-hfq) of S. maltophilia, and compared the behaviour of wild-type and Delta-hfq S. maltophilia cells in a variety of assays. Differential RNA sequencing analysis (dRNA-seq) of RNA isolated from S. maltophilia wild-type and Delta-hfq strains showed that Hfq regulates expression of genes encoding flagellar and fimbrial components, transmembrane proteins, as well as enzymes involved in different metabolic pathways. Moreover, we analysed expression of several sRNAs identified by dRNA-seq in wild-type. The accumulation of two sRNAs was strongly reduced in the absence of Hfq. TEX (terminator exonuclease) treated and untreated libraries of the wild type and the Delta-hfq mutant were sequenced and compared

ORGANISM(S): Stenotrophomonas maltophilia

SUBMITTER: Konrad FM-CM-6rstner 

PROVIDER: E-GEOD-39705 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Functional characterization of the RNA chaperone Hfq in the opportunistic human pathogen Stenotrophomonas maltophilia.

Roscetto Emanuela E   Angrisano Tiziana T   Costa Valerio V   Casalino Mariassunta M   Förstner Konrad U KU   Sharma Cynthia M CM   Di Nocera Pier Paolo PP   De Gregorio Eliana E  

Journal of bacteriology 20120824 21

Hfq is an RNA-binding protein known to regulate a variety of cellular processes by interacting with small RNAs (sRNAs) and mRNAs in prokaryotes. Stenotrophomonas maltophilia is an important opportunistic pathogen affecting primarily hospitalized and immunocompromised hosts. We constructed an hfq deletion mutant (Δhfq) of S. maltophilia and compared the behaviors of wild-type and Δhfq S. maltophilia cells in a variety of assays. This revealed that S. maltophilia Hfq plays a role in biofilm format  ...[more]

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