Tissue Slice Grafts of Human Renal Cell Carcinoma: an Authentic Preclinical Model with High Engraftment Rate and Metastatic Potential

ABSTRACT: Purpose: Discovery of curative therapies for renal cell carcinoma (RCC) is hampered by lack of authentic preclinical models. Tumorgrafts, generated by direct implantation of patient-derived tissues into mice, have demonstrated superior ability to predict therapeutic response. We evaluated “tissue slice grafts” (TSGs) as an improved tumorgraft model of RCC. Experimental Design: Cores of fresh RCC were precision-cut at 300-μm and implanted under the renal capsule of RAG2-/-γC-/- mice. Engraftment rate, histology, biomarker expression, genetic fidelity and metastatic potential were evaluated. Magnetic resonance imaging (MRI) was tested as a non-invasive method to measure tumor volume, and response to a targeted therapy was determined. Results: All 13 cases of RCC engraft

ORGANISM(S): Homo sapiens

SUBMITTER: Hongjuan Zhao 

PROVIDER: E-GEOD-44548 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress