Unknown,Transcriptomics,Genomics,Proteomics

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Transcriptional effect of CRH on MCF7 estrogen receptor signalling


ABSTRACT: MCF7 cells were stimulated with vehicle or 100nM corticotropin releasing hormone (CRH) for 24h. The effect of CRH on the expression of genes relevant to estrogen signalling was investigated by using the Human Estrogen Receptor Signaling RTM-BM-2 ProfilerM-bM-^DM-" PCR Array (SABioscience Corp). qPCR gene expression profiling. MCF7 cells were treated separately in triplicate. Equal amount total RNA was processed further for gene expression analysis.

ORGANISM(S): Homo sapiens

SUBMITTER: dimtiris grammatopoulos 

PROVIDER: E-GEOD-47958 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Estrogen alters the splicing of type 1 corticotropin-releasing hormone receptor in breast cancer cells.

Lal Suchita S   Allan Anna A   Markovic Danijela D   Walker Rosemary R   Macartney James J   Europe-Finner Nick N   Tyson-Capper Alison A   Grammatopoulos Dimitris K DK  

Science signaling 20130702 282


Hormonal stress response is associated with the pathogenesis of disease, including cancer. The role of the stress hormone CRH (corticotropin-releasing hormone) in breast cancer is complex, and its abundance and biological activity may be modulated by estrogen. In the estrogen receptor-positive (ER+) malignant mammary epithelial cell line MCF7, CRH activated numerous kinases and downstream effectors, at least some of which were mediated by the CRH receptor type 1 (CRH-R1). CRH also increased the  ...[more]

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