Project description:To explore possible oncogenic factors in OS, we used a microarray-based approach to profile changes in the expression of mRNAs in five OS cell lines and human mesenchymal stem cells (hMSCs). We used a microarray-based approach to profile the mRNA expression of OS Human OS cell lines were analyzed with genome-wide expression arrays.
Project description:To explore possible oncogenic factors in OS, we used a microarray-based approach to profile changes in the expression of miRNAs in five OS cell lines and human mesenchymal stem cells (hMSCs). We used a microarray-based approach to profile in the miRNA expression of OS Human OS cell lines were analyzed with genome-wide expression arrays.
Project description:To explore possible oncogenic factors in ES, we used a microarray-based approach to profile changes in the expression of miRNAs in five ES cell lines and human mesenchymal stem cells (hMSCs). We used a microarray-based approach to profilein the mRNA expression of ES Human ES cell lines were analyzed with genome-wide expression arrays.
Project description:To explore possible oncogenic factors in ES, we used a microarray-based approach to profile changes in the expression of mRNAs in five ES cell lines and human mesenchymal stem cells (hMSCs). We used a microarray-based approach to profilein the mRNA expression of ES Human ES cell lines were analyzed with genome-wide expression arrays.
Project description:Competent oocytes can be discriminated by BCB staining. Positive stained oocytes are considered more competent than BCB negative oocyte, and injection of BCB+ oocyte extracted mitochondria into BCB negative oocytse can increase fertilisation and blastocyst rate. Here we have analysed the impact of mitochondrial supplementation on subsequent blastocyst transcriptome using agilent one color microarray that is specificly design to study the porcine embryo preimplantation period. Blastocysts were produced by intra cytoplasmic sperm injection (ICSI) from BCB positive and BCB negative oocytes as well as BCB negative oocytes supplemented with mitochondrial extract during ICSI (mICSI), and 3-4 single blatocyst transcriptomes were analysed for each group. 3-4 single blastocysts were analysed at the RNA level after whole transcriptome amplification, and level of gene expression was compared between groups, i.e ICSI BCB+ blastocysts (4), ICSI BCB- blastocysts (3) and mICSI BCB- blastocysts (4).
Project description:To investigate time-dependent changes the comprehensive gene expressions in colorectal normal and tumor surgical specimen within two hours. Both normal and tumor tissues were extracted at 0, 30, 60, 120 min after surgical removal in seven patients with locally advanced colorectal cancer and stabilized. RNA was extracted and calculated, time dependent changes of gene expression were examined by microarray data.
Project description:Inclusion body myopathy associated with Paget disease of bone and frontotemporal dementia (IBMPFD) is caused by mutations in the Valosin Containing Protein (VCP) gene on chromosome 9p12-13. To elucidate affected signaling transduction axes in IBMPFD, we determined expression profiles using microarray technology in quadriceps muscle from patients and unaffected relatives. Muscle from 10 individuals (7 affected, 3 unaffected first degree relatives) was obtained after informed consent for the muscle biopsy was obtained.
Project description:To identify dysregulated molecules between pterygium tissues and uninvolved conjunctiva tissues from the same eye, we performed whole genome microarray expression profiling. Total RNA from four pairs of pterygium and uninvolved conjunctiva tissues from the same eye was extracted and used for microarray experiments.
Project description:Cardiac fibroblasts are critical to proper heart function through multiple interactions with the myocardial compartment. Loosely defined based on their mesenchymal origin, capacity to adhere to plastic and to secrete extracellular matrix, cardiac fibroblasts have been largely neglected due to heterogeneity and lack of proper markers. Objective: To identify genes uniquely expressed in the cardiac fibroblast pool, we performed an unbiased comparative analysis between cardiac and tail fibroblasts, and tracked cardiac fibroblasts after injury to determine their contribution to myocardial regeneration. Methods and Results: High-throughput cell surface and intracellular profiling identified homogeneously expressed MSC markers in cardiac fibroblasts, as well as a surprising number of cardiogenic markers, some expressed at higher levels than in cardiomyocytes. Genetically marked fibroblasts contributed to interstitial but not cardiomyocyte compartments in infarcted hearts. Conclusions: The core transcriptional identity of cardiac fibroblasts reflects their embryological origin, provoking novel interpretations for studies on more specialized cardiac progenitors, and offering a novel perspective for reinterpreting cardiac regenerative therapies 3 biological replicates plated over 5 days after isolation pooled out of 2 animals were used for both tail and heart fibroblasts (6 total samples analysed)