Transcription profiling of mouse thymus gland from pre-diabetic and diabetic animals.

ABSTRACT: Female NOD mice were born in specific pathogen free (SPF) conditions at the CEMIB-UNICAMP animal facility (University of Campinas, S�o Paulo, Brazil) and maintained in SPF mini-isolators in our laboratory at the University of S�o Paulo, Campus of Ribeir�o Preto, Brazil. We studied both pre-diabetic (8�2 week-old) and diabetic (20�2 week-old) animals. Diabetes was confirmed by blood glucose levels (?250 mg glucose/dL) using the Accu Check � Active Kit (Roche Diagn�stica Brasil, S�o Paulo, Brazil). The thymic stroma was separated from the whole thymus. The mTEC 3.10 medullary thymic epithelial cell line was established from C57BL/6 mice, and the original medullary phenotype was confirmed by immunostaining with anti-cytokeratin monoclonal antibodies. In addition, the CD80+ phe

ORGANISM(S): Mus musculus

DISEASE(S): type 1 diabetes mellitus (DM-1)

SUBMITTER: Geraldo Passos 

PROVIDER: E-MEXP-2339 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress