Browse
Submit Data
Databases
API
Help

Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

8 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Transcription profiling by array of Arabidopsis thaliana plants to investigate the effect of heterologous expression of a soybean DREB2 gene (GmDREB2A;2)

ABSTRACT: Analysis of the effect of heterologous expression of a soybean DREB2 gene (GmDREB2A;2)on the transcriptome of transgenic Arabidopsis plants. For the genetic modification of Arabidopsis, we introduced gene casettes that express our genes of interest into the genome viaT-DNA transfer using Agrobacterium tumefaciens.

ORGANISM(S): Arabidopsis thaliana

SUBMITTER: Junya Mizoi

PROVIDER: E-MEXP-3692 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

ACCESS DATA

Json Xml

Publications

GmDREB2A;2, a canonical DEHYDRATION-RESPONSIVE ELEMENT-BINDING PROTEIN2-type transcription factor in soybean, is posttranslationally regulated and mediates dehydration-responsive element-dependent gene expression.

Mizoi Junya J Ohori Teppei T Moriwaki Takashi T Kidokoro Satoshi S Todaka Daisuke D Maruyama Kyonoshin K Kusakabe Kazuya K Osakabe Yuriko Y Shinozaki Kazuo K Yamaguchi-Shinozaki Kazuko K

Plant physiology 20121114 1

Soybean (Glycine max) is an important crop around the world. Abiotic stress conditions, such as drought and heat, adversely affect its survival, growth, and production. The DEHYDRATION-RESPONSIVE ELEMENT-BINDING PROTEIN2 (DREB2) group includes transcription factors that contribute to drought and heat stress tolerance by activating transcription through the cis-element dehydration-responsive element (DRE) in response to these stress stimuli. Two modes of regulation, transcriptional and posttransl ...[more]

PMID: 23151346

Similar Datasets

Downstream analysis of DREB2A and DREB2B

Project description:Transcriptome analysis of transgenic Arabidopsis overexpressing a constitutive active form of DREB2A or DREB2B

2023-12-30 | E-MEXP-3606 | biostudies-arrayexpress

Genome-wide gene expression analysis of DEWAX OX plant lines compared to wild type

Project description:The experiment was designed to enable comparison between columbia and DEWAX OX plants line Arabidopsis stems

2013-12-01 | E-MEXP-3781 | biostudies-arrayexpress

Transcription profiling by array of Arabidopsis thaliana columbia and DEWAX2 overexpression plants line stems

Project description:The experiment was designed to enable comparison between Arabidopsis thaliana columbia and DEWAX2 OX plants line.

2017-12-31 | E-MTAB-5980 | biostudies-arrayexpress

Expression data of Arabidopsis overexpressing ERF96 gene

Project description:Arabidopsis overexpressing ERF96 are more resistant against necrotrophic pathogen such as Botrytis cinerea. ERF96 is a member of the AP2/ERF superfamily of transcription factor. We used Microarray to determine which gene expressions are up-regulated due to an accumulation of ERF96 and to identify the putative direct target of this transcription factor. Microarray were performed from the RNA of a four-week old Arabidopsis overexpressing ERF96 and WT(col-0). RNA was extracted during three independant experiments

2014-07-31 | E-GEOD-58231 | biostudies-arrayexpress

Microarray analysis of transcriptional responses to abscisic acid and salt stress in Arabidopsis thaliana

Project description:To investigate differences in plant responses to salt and ABA stimulus, differences in gene expression in Arabidopsis in response to salt and ABA were compared using an Agilent oligo microarray. Four-week-old Arabidopsis thaliana ecotype Columbia (Col-0) seedlings were treated with either 150 mM NaCl or 10 M-NM-<M ABA for 6 hours; unstressed seedlings (control sample) were collected in parallel to avoid the possible effects of circadian rhythms. The results revealed that 31 genes were up regulated by both NaCl and ABA stress, and 23 genes were down-regulated by these stressors. To provide further validation of our microarray experiment data, ten genes from this signature were quantified in the same RNA samples by quantitative real-time PCR. Differentially expression genes of Arabidopsis thaliana were measured under salt stressed, ABA stressed and normal condition for 6 hours, respectively. Three independent experiments were performed at each treatment using different plants for each experiment.

2013-03-28 | E-GEOD-45543 | biostudies-arrayexpress

Identification of target genes of ABI3 [Agilent 2x244k]

Project description:ABI3 is a key regulator of seed development in Arabidopsis and other plants.To identify DNA fragments(promoters) bound by ABI3 we performed array based ChIP analysis. Two ChIP experiments were performed with chromatin of seeds of Arabidopsis and an anti-ABI3 antibody directed against a N-terminal fragment of ABI3, raised in rabbits. Non precipitated chromatin (input) served as control.

2012-05-31 | E-GEOD-34033 | biostudies-arrayexpress

Transcriptome profiling of LecRKVI.2 over-expressor plants.

Project description:The arabidopsis L-type lectin receptor kinase-VI.2 positively regulates bacterial PAMP-triggered immunity. We used microarrays and performed genome-wide transcriptome analysis of LecRKVI.2 over-expressing plants and identified number of upregulated genes involved against bacterial resistance. Total RNA was isolated from 5-week-old Arabidopsis plants for RNA extraction and hybridisation on Affymetrix microarrays.

2012-03-19 | E-GEOD-26646 | biostudies-arrayexpress

Transcription profiling of Arabidopsis mkk1, mkk2 and mkk1/2 knockout treated with the saliicylic acid analog BTH

Project description:Gene Expression profiling of the Arabidopsis thaliana MAP Kinase Kinases 1 (mkk1), MAP Kinase Kinases 2 (mkk2) knockout mutants and the double mutant mkk1/mkk2 before and 24 hours after treatment with the salicylic acid analog BTH, was measured by hybridisation to an Affymetrix ATH1 GeneChip. Experiment Overall Design: Whole Arabidopsis thaliana mkk1, mkk2 and mkk1/mkk2 knockout mutants and wild type plants (Col-0) were sampled in triplicates before and 24 hours after BTH treatment.

2008-06-11 | E-GEOD-10646 | biostudies-arrayexpress

Identification of mir-342-3p functionally associated with transcriptional activity of MYC in lung cancer [patients]

Project description:Accumulating evidence indicates that altered miRNA expression is crucially involved in lung cancer development, but only little has been elucidated about how MYC, an archetypical oncogene, is regulated by miRNAs, especially through a mechanism involving the MYC cofactor(s). In this study, we aimed at identifying miRNAs involved in the regulation of MYC transcriptional activity in lung cancers. To this end, we have taken an integrative approach with combinatorial usage of miRNA and mRNA expression profile datasets of patientsâ tumor tissues as well as those of MYC-inducible cell lines in vitro. We report here that in addition to miRNAs previously reported to be directly regulating or regulated by MYC including let-7 and miR-17-92, our strategy allowed us to identify miR-342-3p as the one capable of indirectly regulating MYC activity through direct repression of a MYC-cooperating molecule, E2F1. Furthermore, the miR-342-3p module activity, which we defined as a gene set reflecting experimentally substantiated influence of miR-342-3p on mRNA expression, was found to be inversely correlated with MYC activity reflected in the MYC module activity in three independent datasets of lung adenocarcinoma patients obtained by the Directorâs Challenge Consortium of the United States (P=1.94x10-73), the National Cancer Center of Japan (P=9.05x10-34) and our own in this study (P=3.37x10-6). Our integrative approach thus appears to be useful to elucidate inter-regulatory relationships between miRNAs and a protein coding-gene of interest, even those present in tumor tissues in patients, which still remains a challenge to better understand the pathogenesis of this devastating disease. Microarray analysis using a SurePrint G3 Human GE 8 x 60K Microarray G4851A (Agilent) was conducted.

2016-01-06 | E-GEOD-66759 | biostudies-arrayexpress

Transcription profiling by array of A. thaliana columbia stems after treatment of hypoxia to investigate genome-wide gene expression analysis

Project description:The experiment was designed to enable comparison between Arabidopsis stems treated hypoxia and Arabidopsis stems treated normoxia.

2015-12-31 | E-MTAB-3510 | biostudies-arrayexpress

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data