Transcription profiling of Arabidopsis plants inducibly expressing LEC2:GR or ttg:GR to identify primary and secondary targets of the LEC2 transcription factor
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ABSTRACT: Comparison of various inductibles constructions of arabidopsis treated by differents solutions. The aim of this project is to determine the primary and secondary targets of the LEC2 transcrition factor. The induction by the dexamethasone allows the expression of constructions LEC2:GR or ttg:GR introduced into transforming plants.The dexamethasone induces the expression of the contruct which carries the receptor to the glucocorticoïde (GR.). The cycloheximide is a inhibitor of the translation and allows, coupled with the dexamethasome, the identification of the primary targets only. The LEC2_GR25 and LEC2_GR31 constructs are two independents transforming plants of the cDNA-LEC2_GR construct. Both were used in order to have an idea of the variability of the data transcriptome obtained. The ttg_GR construct is used here as control ; ttg is another transcription factor, thus if induction with the dexamethasone works well, the targets induced in the two mutants will be different.
ORGANISM(S): Arabidopsis thaliana
SUBMITTER: Sandrine balzergue
PROVIDER: E-MEXP-807 | biostudies-arrayexpress |
REPOSITORIES: biostudies-arrayexpress
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