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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Transcriptional regulation of endocrine cell differentiation by Insm1

ABSTRACT: This experiment used RNA-Seq technology to explore gene expression in mouse Insm1^GFP/Pdx1^CFP HIGH [het/het] FACS sorted pancreatic cells (pre-beta cells) and Insm1^GFP/Pdx1^CFP LOW [het/het] cells (other endocrine progenitors) at E15.5 and E18.5. Comparison of Insm1 +/Pdx HIGH and Insm1 +/Pdx LOW cells revealed a set of differentially expressed genes that are required for beta cell specification.

INSTRUMENT(S): Illumina HiSeq 2000

ORGANISM(S): Mus musculus

SUBMITTER: Mark Magnuson

PROVIDER: E-MTAB-1929 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Insm1 promotes endocrine cell differentiation by modulating the expression of a network of genes that includes Neurog3 and Ripply3.

Osipovich Anna B AB Long Qiaoming Q Manduchi Elisabetta E Gangula Rama R Hipkens Susan B SB Schneider Judsen J Okubo Tadashi T Stoeckert Christian J CJ Takada Shinji S Magnuson Mark A MA

Development (Cambridge, England) 20140801 15

Insulinoma associated 1 (Insm1) plays an important role in regulating the development of cells in the central and peripheral nervous systems, olfactory epithelium and endocrine pancreas. To better define the role of Insm1 in pancreatic endocrine cell development we generated mice with an Insm1(GFPCre) reporter allele and used them to study Insm1-expressing and null populations. Endocrine progenitor cells lacking Insm1 were less differentiated and exhibited broad defects in hormone production, ce ...[more]

PMID: 25053427

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