Deep sequencing of human naive CD4 T cells identifies miR-34c-5p as a novel regulator of T cell activation and HIV replication

ABSTRACT: Cell activation is a vital step for T cell memory/effector differentiation as well as for productive HIV infection. To identify novel regulators of this process, we used next generation sequencing to profile changes in microRNA expression occurring in purified human naive CD4 T cells in response to TCR stimulation and HIV infection. HIV infection had no significant impact on global miR expression in quiescent nave CD4 T cells. We identified miR-34c-5p as a novel miR strongly induced by TCR stimulation of nave CD4 T cells, and found that it was consistently down-regulated in response to viral infection. Over-expression of miR-34c-5p had a positive effect on HIV-1 replication. Finally, we demonstrated that miR-34c-5p alters the expression of several genes involved in TCR signaling and cell a

INSTRUMENT(S): Illumina Genome Analyzer II, Illumina HiSeq 2000

ORGANISM(S): Homo sapiens

SUBMITTER: Andreia Amaral 

PROVIDER: E-MTAB-4214 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress