Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

0

Characterisation of an oesophageal adenocarcinoma cell line (The MFD-1)


ABSTRACT: A cell line (MFD-1) was derived from a 55-year old male with oesophageal adenocarcinoma. Using different sources of genetic material from normal and tumour tissue surgically resected, peripheral blood and the derived cell line a high concordance of genotypes calls across the whole genome confirms MFD-1 was derived from parent tumour. The SNP6 array contained 906,000 probes for the genotyping of SNPs and 946,000 probes for the genotyping of non-polymorphic copy number. Affymetrix CEL files were analysed using the tool PICNIC2 (predicting absolute allele copy number variation with microarray cancer data).

ORGANISM(S): Homo sapiens

SUBMITTER: Edwin Garcia 

PROVIDER: E-MTAB-4590 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

altmetric image

Publications


New biological tools are required to understand the functional significance of genetic events revealed by whole genome sequencing (WGS) studies in oesophageal adenocarcinoma (OAC). The MFD-1 cell line was isolated from a 55-year-old male with OAC without recombinant-DNA transformation. Somatic genetic variations from MFD-1, tumour, normal oesophagus, and leucocytes were analysed with SNP6. WGS was performed in tumour and leucocytes. RNAseq was performed in MFD-1, and two classic OAC cell lines F  ...[more]

Similar Datasets

2015-01-03 | E-MTAB-2694 | biostudies-arrayexpress
2016-09-09 | E-MTAB-4600 | biostudies-arrayexpress
2010-01-08 | E-MEXP-2463 | biostudies-arrayexpress
2011-07-01 | E-GEOD-30348 | biostudies-arrayexpress
2012-04-28 | E-GEOD-34676 | biostudies-arrayexpress
2013-02-01 | E-GEOD-37259 | biostudies-arrayexpress
2007-03-21 | E-TABM-155 | biostudies-arrayexpress
2016-05-18 | E-GEOD-69614 | biostudies-arrayexpress
2010-06-09 | E-GEOD-20584 | biostudies-arrayexpress
2011-08-01 | E-MTAB-624 | biostudies-arrayexpress