DNA methylation profiling of primary human CD4+T-cell memory subsets

ABSTRACT: Differentiation of CD4+T-cells into effector subsets is a critical component of the adaptive immune system and an incorrect response can lead to autoimmunity or immune deficiency. Cellular differentiation including T-cell differentiation is accompanied by large-scale epigenetic remodeling, including changes in DNA methylation at key regulators of T-cell differentiation. The TET family of enzymes were recently shown to be able to catalyse methylated cytosine (5mC) into 5-hydroxymethylcytosine (5hmC) enabling a pathway of active removal of DNA methylation. Here, we characterize 5hmC, 5mC and transcriptional dynamics during human CD4+T-cell polarisation in a time series approach and relate these changes to profiles in ex-vivo CD4+memory subsets. We observed large-scale remodelling during earl

INSTRUMENT(S): Illumina iScan

ORGANISM(S): Homo sapiens

SUBMITTER: Antonio Lentini 

PROVIDER: E-MTAB-4689 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress