The global effect of the garlic compound Allicin on the changes in the transcriptome in Bacillus subtilis and Staphylococcus aureus USA300 analysed by RNA-seq of coding RNA

ABSTRACT: To monitor the global changes in gene expression of Staphylococcus aureus USA300 TCH1516 under exposure to the garlic compound Allicin by RNA-seq, cultivation was performed in shaking flasks in Luria Bertani (LB) medium in triplicate at 37C until cells have reached an optical density at 540nm of 2.0. Cells were harvested by centrifugation, washed with Belitsky minimal medium (BMM) and adapted to BMM for one hour before exposure to 300 M Allicin stress. S. aureus cells of 3 replicate experiments were harvested before and 30 min after exposure to 300 M Allicin and disrupted in 3 mM EDTA/ 200 mM NaCl lysis buffer with a Precellys24 Ribolyzer. RNA isolation was performed using the phenol-chloroform-isoamylalcohol approach. After precipitation with 3 M sodium acetate and isopropanol, the total

INSTRUMENT(S): Illumina MiSeq

ORGANISM(S): Staphylococcus aureus subsp. aureus USA300

SUBMITTER: Loi Vu Van 

PROVIDER: E-MTAB-5667 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress