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Rilkit-eqtl analysis (random pair design). Tools to optimize the use of RIL populations for natural diversity studies and QTL cloning

ABSTRACT: gnp07_rilkit - rilkit-eqtl analysis (random pair design). To provide quantitative geneticists studying natural variation in our RIL populations with information and resources necessary to speed up QTL cloning and candidate genes identification. This articulates around the creation of two major resources and their integration into databases to make the information accessible to users: A. Whole transcriptome survey, B. Production of complementary material. Comparison of 164 Cvi/Col(8RV) Recombinant Inbred Lines (RILs) and 164 Bur/Col (20RV) RILs.

ORGANISM(S): Arabidopsis thaliana

PROVIDER: GSE28791 | GEO | 2011/06/30

SECONDARY ACCESSION(S): PRJNA138825

REPOSITORIES: GEO

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rilkit-eqtl analysis (random pair design). Tools to optimize the use of RIL populations for natural diversity studies and QTL cloning

Project description:gnp07_rilkit - rilkit-eqtl analysis (random pair design). To provide quantitative geneticists studying natural variation in our RIL populations with information and resources necessary to speed up QTL cloning and candidate genes identification. This articulates around the creation of two major resources and their integration into databases to make the information accessible to users: A. Whole transcriptome survey, B. Production of complementary material. Comparison of 164 Cvi/Col(8RV) Recombinant Inbred Lines (RILs) and 164 Bur/Col (20RV) RILs. 162 dye-swaps. Genotype and ecotype comparison.

2011-06-30 | E-GEOD-28791 | biostudies-arrayexpress

Proximal methylation features associated with nonrandom changes in gene body methylation

Project description:Gene body methylation at CG dinucleotides is a widely conserved feature of methylated genomes, but remains poorly understood. The A. thaliana strain Cvi has depleted methylation in gene bodies relative to the common reference strains Col and Ler. This naturally occurring hypomethylated strain offers the opportunity to improve understanding of the function and heritablity of genic CG methylation. We bisulfite-sequenced 10 recombinant inbred lines derived from a cross between Col and Cvi, as well as both parent lines, and examined the transmission of the different Col- and Cvi-derived gene body methylation states in these lines. We found that the majority of CG methylation polymorphisms were faithfully transmitted over nine generations according to the parental genotype in the RILs, with only 1-4% of CGs failing to maintain the parental methylation state. We found no evidence to suggest that these differences in gene body methylation led to changes in gene expression. However, we found that sites that fail to maintain the parental methylation state are nonrandom and are associated with intermediate levels of CG methylation and high methylation variability across many A. thaliana strains. These results provide new insights into the features determining the inheritance of gene body methylation and demonstrate that two different methylation equilibria can be maintained within single individuals.

2017-03-29 | GSE93968 | GEO

Transcriptome profiling of ozone responses of Arabidopsis thaliana natural accessions Col-0 and Cvi-0, and a near-isogenig line Col-S with Cvi-0's ozone sensitivity introgressed into Col-0

Project description:Many Arabidopsis thaliana accession show sensitvity to the air pollutant ozone, including the accession Cvi-0 from the Cape Verde Islands. To understand and assist in genetic mapping of loci causing the ozone sensitvity of Cvi-0, transcript profiling was performed in Cvi-0, the tolerant Col-0, and a near isogenic line (Col-S) where ozone sensitivity was introgressesed from Cvi-0 to Col-0 through eight rounds of backcrossing.

2017-08-09 | GSE102363 | GEO

h3k27me3_col(cvi)-Analysis of epigenomic changes in hybrids Arabidopsis thaliana Col-0, C24 and Cvi accessions

Project description:a2e_heterosis - h3k27me3_col(cvi) - Arabidopsis thaliana accessions (Col-0, C24 and Cvi) and their hybrid were used to investigate the dynamics of the epigenome after intraspecific hybridization between - H3K27me3 profiling in Col

2011-05-17 | GSE25052 | GEO

h3k4me2_col(cvi)-Analysis of epigenomic changes in hybrids Arabidopsis thaliana Col-0, C24 and Cvi accessions

Project description:a2e_heterosis - h3k4me2_col(cvi) - Arabidopsis thaliana accessions (Col-0, C24 and Cvi) and their hybrid were used to investigate the dynamics of the epigenome after intraspecific hybridization between - H3K4me2 profiling in Col-0

2011-05-17 | GSE25056 | GEO

cgh_colvscvi_wg-Analysis of epigenomic changes in hybrids Arabidopsis thaliana Col-0, C24 and Cvi accessions

Project description:a2e_heterosis - cgh_colvscvi_wg - Arabidopsis thaliana accessions (Col-0, C24 and Cvi) and their hybrid were used to investigate the dynamics of the epigenome after intraspecific hybridization between - Comparative genome hybridization between Arabidopsis thaliana accessions Col-0 and CVi.

2011-05-17 | GSE24835 | GEO

Analysis of epigenomic changes in hybrids Arabidopsis thaliana Col-0, C24 and Cvi accessions

Project description:This SuperSeries is composed of the following subset Series: GSE24494: cgh_colvsc24_chr4-Analysis of epigenomic changes in hybrids Arabidopsis thaliana Col-0, C24 and Cvi accessions GSE24831: cgh_colvsc24_wg-Analysis of epigenomic changes in hybrids Arabidopsis thaliana Col-0, C24 and Cvi accessions GSE24835: cgh_colvscvi_wg-Analysis of epigenomic changes in hybrids Arabidopsis thaliana Col-0, C24 and Cvi accessions GSE25049: h3k4me2_c24_chr4-Analysis of epigenomic changes in hybrids Arabidopsis thaliana Col-0, C24 and Cvi accessions GSE25050: h3k27me3_colxcvi-Analysis of epigenomic changes in hybrids Arabidopsis thaliana Col-0, C24 and Cvi accessions GSE25051: h3k27me3_cvi-Analysis of epigenomic changes in hybrids Arabidopsis thaliana Col-0, C24 and Cvi accessions GSE25052: h3k27me3_col(cvi)-Analysis of epigenomic changes in hybrids Arabidopsis thaliana Col-0, C24 and Cvi accessions GSE25053: h3k4me2_colxcvi-Analysis of epigenomic changes in hybrids Arabidopsis thaliana Col-0, C24 and Cvi accessions GSE25054: h3k4me2_cvi-Analysis of epigenomic changes in hybrids Arabidopsis thaliana Col-0, C24 and Cvi accessions GSE25056: h3k4me2_col(cvi)-Analysis of epigenomic changes in hybrids Arabidopsis thaliana Col-0, C24 and Cvi accessions GSE25057: h3k27me3_colxc24-Analysis of epigenomic changes in hybrids Arabidopsis thaliana Col-0, C24 and Cvi accessions GSE25058: h3k27me3_c24-Analysis of epigenomic changes in hybrids Arabidopsis thaliana Col-0, C24 and Cvi accessions GSE25059: h3k27me3_col(c24)-Analysis of epigenomic changes in hybrids Arabidopsis thaliana Col-0, C24 and Cvi accessions GSE25060: h3k4me2_colxc24_chr4-Analysis of epigenomic changes in hybrids Arabidopsis thaliana Col-0, C24 and Cvi accessions GSE25061: h3k4me2_col_chr4-Analysis of epigenomic changes in hybrids Arabidopsis thaliana Col-0, C24 and Cvi accessions Refer to individual Series

2011-05-17 | E-GEOD-24836 | biostudies-arrayexpress

h3k4me2_cvi-Analysis of epigenomic changes in hybrids Arabidopsis thaliana Col-0, C24 and Cvi accessions

Project description:a2e_heterosis - h3k4me2_cvi - Arabidopsis thaliana accessions (Col-0, C24 and Cvi) and their hybrid were used to investigate the dynamics of the epigenome after intraspecific hybridization between - H3K4me2 profiling in Cvi

2011-05-17 | GSE25054 | GEO

h3k27me3_cvi-Analysis of epigenomic changes in hybrids Arabidopsis thaliana Col-0, C24 and Cvi accessions

Project description:a2e_heterosis - h3k27me3_cvi - Arabidopsis thaliana accessions (Col-0, C24 and Cvi) and their hybrid were used to investigate the dynamics of the epigenome after intraspecific hybridization between - H3K27me3 profiling in Cvi seedlings

2011-05-17 | GSE25051 | GEO

h3k4me2_col_chr4-Analysis of epigenomic changes in hybrids Arabidopsis thaliana Col-0, C24 and Cvi accessions

Project description:a2e_heterosis - h3k4me2_col_chr4 - Arabidopsis thaliana accessions (Col-0, C24 and Cvi) and their hybrid were used to investigate the dynamics of the epigenome after intraspecific hybridization between - H3K4me2 profiling in Col

2011-05-17 | GSE25061 | GEO

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