Ontology highlight
ABSTRACT:
ORGANISM(S): Mus Musculus
SUBMITTER: Ping Xu
PROVIDER: PXD012738 | iProX | Fri Feb 15 00:00:00 GMT 2019
REPOSITORIES: iProX
Rapid communications in mass spectrometry : RCM 20190901 17
<h4>Rationale</h4>Formalin-Fixed Paraffin-Embedded (FFPE) samples are valuable for proteomic studies of disease. However, the crosslink among proteins, protein vs nucleic acid, and other covalent chemical modifications like methylation introduced by formaldehyde can interfere with trypsin digestion in proteomics studies. LysargiNase was reported to have a better full-cleavage rate at methylation and b ion coverage than trypsin. The contribution of LysargiNase in the proteomic study of FFPE sampl ...[more]