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Proteome analyses of two ovarian clear cell carcinoma cell lines with or without ARID1A manipulation

ABSTRACT: ARID1A, which encodes a component of the SWI/SNF chromatin-remodeling complex, is commonly mutated in ovarian clear cell carcinoma and many other cancer types. We used label-free LC-MS/MS to identify ARID1A-dependent proteome changes in ovarian clear cell carcinoma cell lines. In our first analysis, we compared ARID1A-wildtype ovarian clear cell carcinoma cell line OVCA429 with or without ARID1A CRISPR knockout. In a complementary analysis, we compared ARID1A-mutated ovarian clear cell carcinoma cell line OVISE with or without ARID1A overexpression using a tet-inducible promoter.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Homo Sapiens (ncbitaxon:9606)

SUBMITTER: David W. Speicher

PROVIDER: MSV000079904 | MassIVE | Wed Jul 13 05:35:00 BST 2016

SECONDARY ACCESSION(S): PXD004570

REPOSITORIES: MassIVE

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