Project description:Soluble proteins of Shingobium sp. strain Chol11 adapted to growth with cholate were compared to cells adapted to growth with glucose by means of 2D DIGE coupled to MALDI-TOF MS protein identification
Project description:With the aim of reveal the mechanism of Podophyllum Hexandrum Royle adaption to high altitude, MALDI-TOF-MS/MS in combination with 2-DE was used to identify the differentially expressed proteins in leaves of Podophyllum Hexandrum Royle between low altitude (2200m) and high altitude (3100m), 65 differentially expressed proteins was identifed and 44 was up-regulated in high altitude compared tolow altitude.
Project description:Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) has exhibited advantages in rapid analysis of metabolites. This data set provides support to the discuss of influence of interferential species, matrix effect and instrument parameters on metabolite qualification and quantification.
Project description:This bacterial MALDI-TOF dataset was used for the study "AMinimizing Taxonomic and Natural Product Redundancy in Microbial Libraries Using MALDI-TOF MS and the Bioinformatics Pipeline IDBac"
It consists of triplicate MALDI-TOF MS profiles (both reflectron and linear datasets) for >1,600 bacteria cultivated from a collection in Iceland.
Project description:It is of high importance to distinguish Tilletia caries and Tilletia laevis as causal agents of common bunt accurately from Tilletia controversa, the causal agent of dwarf bunt. All three of these wheat bunt diseases can lead to significant yield losses in crop production worldwide. But T. controversa is categorized as a quarantine pest in most areas of the world and must be discriminated from the T. caries / T. laevis complex. Usually, morphological characteristics of the teliospores are used to differentiate the three species. But due to natural hybridization and overlapping properties the discrimination is challenging. Germination behavior can also be considered for discrimination, but equivalent to their similar physiological and genetic traits the two agents of common bunt, T. caries and T. laevis could not be distinguished by this. It was suggested that the two species and maybe all three of those described Tilletia species might be conspecific. Up to now no molecular based method is available to differentiate the three species. Several studies have attempted the detection of the wheat bunt Tilletia species using PCR or other DNA-based methods. Other studies analyzed protein patterns with electrophoresis methods. But none of these approaches was able to distinguish between all of the three closely related Tilletia species. Several studies have shown that Matrix-Assisted Laser Desorption/Ionization-Time of Flight Mass Spectrometry (MALDI-TOF MS) is a useful tool to differentiate closely related fungal species. The aim of this study was to assess whether MALDI-TOF MS analysis is able to distinguish specimens of the three closely related pathogens T. caries, T. laevis, and T. controversa and may constitute an alternative method to the usually used morphology-based identification. Therefore MALDI-TOF MS was used to create subproteome fingerprints of the teliospores of 69 Tilletia specimens. These fingerprints were analyzed by comparing the mass spectra to each other by high-throughput multidimensional scaling (HiT-MDS ) together with hierarchical cluster analysis (HCA). The second approach was performed by discriminant analysis of principal components (DAPC). MALDI-TOF MS has proven to be a useful method for distinguishing between T. controversa and the two causal agents of common bunt, using our developed method of direct analysis of teliospores, but was unable to separate T. caries and T. laevis species. We conclude a potentially conspecific status of T. caries and T. laevis or even two morphotypes of one common species, causing identical disease symptoms and sharing the same germination requirements along with a related protein composition, shown in this study. Our developed MALDI-TOF MS method can be helpful in testing Tilletia bunt balls collected during field inspections, especially with regard to quarantine regulations or for breeding applications and may also be transferred to analyze further challenging sample material.
2022-02-14 | PXD030401 | Pride
Project description:MALDI-TOF MS as a useful tool for rapid identification of flea vectors preserved in alcohol from field-collected samples.
Project description:MALDI-TOF MS analysis was performed using an Autoflex Speed LRF mass
spectrometer (Bruker Daltonics) equipped with a Smartbeam-II laser (355 nm).
Project description:To expand the knowledge on the porcine salivary proteome, secretions from the three major salivary glands were collected from anaesthetised piglets. Pilocarpine and isoproterenol were simultaneously injected intraperitoneally to increase the volume and protein concentration of the saliva, respectively. The protein composition and relative protein-specific abundance of saliva secreted by the parotid gland and by the mandibular and monostomatic sublingual gland, were determined using iTRAQ. When combining two detection methods, MALDI-TOF/TOF MS and Q-Exactive orbitrap MS/MS, a total of 122 porcine salivary proteins and 6 mammalian proteins with a predicted porcine homolog were identified. Only a quantitative and not a qualitative difference was observed between both ductal secretions. The 128 proteins were detected in both secretions, however, at different levels. Twenty-four proteins (20 porcine, 4 mammalian with a predicted porcine homolog) were predominantly secreted by the parotid gland, such as carbonic anhydrase VI and alpha-amylase. Twenty-nine proteins (all porcine) were predominantly secreted by the mandibular and sublingual glands, for example salivary lipocalin and submaxillary apomucin protein.
Project description:To construct a rapid, high-throughput screening method for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection based on matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS).