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Genome-wide maps of E2A binding peaks in Embryonic stem cells and Neural progenitor cells

ABSTRACT: Genome-wide maps of E2A binding peaks in Embryonic stem cells and Neural progenitor cells

PROVIDER: PRJNA646461 | ENA |

REPOSITORIES: ENA

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Genome-wide maps of E2A binding peaks in Embryonic stem cells and Neural progenitor cells

Project description:Background: E2A, encoded by the TCF3 gene locus, belongs to the E protein transcription factor family, which also includes HEB (TCF12) and E2-2 (TCF4), has been suggested to play an important role in leukemogenesis. However, far less is known about the function of E2A in cell-fate regulation of hESCs. Therefore, further understanding of E2A in self-renewal and differentiation of embryonic stem cells may be influenced. Methods: The mRNA profiles of wildtype and E2A knockout embryonic stem cells were generated by RNA-seq technique, in triplicate for each group, using IIIumina Hiseq 2500. Results: A comprehensive human transcriptome map of wild type and E2A knockout embryonic stem cells was provided. Function enrichment, network characteristics and disease association of the differentially expressed genes were analyzed. Conclusion: The dataset could serve as a baseline resource for investigating the potential effects and mechanism of E2A in embryonic stem cells.

2020-07-16 | GSE154479 | GEO

Genome-wide maps of chromatin state in wild type and E2A knockout neural progenitor cells

Project description:Background: E2A, encoded by the TCF3 gene locus, belongs to the E protein transcription factor family, which also includes HEB (TCF12) and E2-2 (TCF4), has been suggested to play an important role in leukemogenesis. However, far less is known about the function of E2A in cell-fate regulation of hESCs. Therefore, further understanding of E2A in self-renewal and differentiation of embryonic stem cells may be influenced. In the study, we demonstrated E2A knockout exhibited blocked neural differentiation, which is tightly related to histone modification H3K4me3 and H3K27me3. Methods: The genomic DNA of H3K4me3 and H3K27me3 binding peaks in wild type and E2A knockout neural progenitor cells were generated by ChIP-seq technique using IIIumina Hiseq 2500. Results: A comprehensive human chromatin state of H3K4me3 and H3K27me3 in wild type and E2A knockout neural progenitor cells was provided. Function enrichment, network characteristics and disease association of the binding peaks were analyzed. Conclusion: The dataset could serve as a baseline resource for investigating the potential effects and mechanism of H3K4me3/H3K27me3/E2A complex in neural differentiation period of embryonic stem cells

2020-07-16 | GSE154480 | GEO

Transcriptome profiling of wildtype and E2A knockout embryonic stem cells and Neural progenitor cells

2020-07-16 | GSE154482 | GEO

Genome-wide maps of chromatin state in wild type and E2A knockout neural progenitor cells

Project description:Genome-wide maps of chromatin state in wild type and E2A knockout neural progenitor cells

| PRJNA646462 | ENA

Transcriptome profiling of wildtype and E2A knockout embryonic stem cells and Neural progenitor cells

Project description:Transcriptome profiling of wildtype and E2A knockout embryonic stem cells and Neural progenitor cells

| PRJNA646464 | ENA

Transcriptome profiling of wildtype and E2A knockout embryonic stem cells

2020-07-16 | GSE154481 | GEO

Expression data from WT and E2A-KO bone marrow stem and progenitor cell subfractions.

Project description:Expression profiling of WT and E2A-KO LSK FLT3- and LMPP protenitor cells.

2008-08-31 | GSE11982 | GEO

Transcription profiling of mouse WT and E2A-KO bone marrow stem and progenitor cell subfractions.

Project description:Expression profiling of WT and E2A-KO LSK FLT3- and LMPP protenitor cells. Experiment Overall Design: LSK FLT3- and LMPP stem/progenitor cells from WT and E2A-KO mice were FACS sorted. Subsequently RNA was extracted, labelled and hybridized to Affymetrix microarrays. Goal of experiment was to investigate expression changes between WT and KO LMPP cells.

2008-10-25 | E-GEOD-11982 | biostudies-arrayexpress

Expression data from WT and E2A-deficient murine DN2 cells

Project description:The E2A transcription factors promote the development of thymus-seeding cells but it remains unknown whether these proteins play a role in T lymphocyte lineage specification or commitment. By examining E2A-dependent genes in developing T cells, we will address whether these proteins are involved in these processes. DN2 cells from WT and E2A-deficient murine fetal thymi were FACS sorted. Subsequently RNA was extracted, labelled and hybridized to Affymetrix microarrays. The goal of this study is to investigate E2A-dependent genes in developing T cells

2013-02-01 | E-GEOD-43224 | biostudies-arrayexpress

RNA-sequencing of E2a-deficient or E2a and Lef1-deficient leukemia cell lines

Project description:We compared the transcriptome of leukemia cell lines generated from 7 E2a-/- and 8 E2a-/-Lef1f/fLckCre+ mice

2021-10-25 | GSE186420 | GEO