Ontology highlight
ABSTRACT:
INSTRUMENT(S): LTQ Orbitrap Velos
ORGANISM(S): Mus Musculus (mouse)
SUBMITTER: Hesse Anne-Marie
PROVIDER: PXD000052 | Pride | 2013-08-28
REPOSITORIES: Pride
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Bounab Yacine Y Hesse Anne-Marie- AM Iannascoli Bruno B Grieco Luca L Couté Yohann Y Niarakis Anna A Roncagalli Romain R Lie Eunkyung E Lam Kong-Peng KP Demangel Caroline C Thieffry Denis D Garin Jérôme J Malissen Bernard B Daëron Marc M
Molecular & cellular proteomics : MCP 20130702 10
We report the first proteomic analysis of the SLP76 interactome in resting and activated primary mouse mast cells. This was made possible by a novel genetic approach used for the first time here. It consists in generating knock-in mice that express signaling molecules bearing a C-terminal tag that has a high affinity for a streptavidin analog. Tagged molecules can be used as molecular baits to affinity-purify the molecular complex in which they are engaged, which can then be studied by mass spec ...[more]