Proteomics

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SLP76 interactome in resting and activated primary mast cells


ABSTRACT: We used a knock-in (KI) mouse expressing SLP-76 with a C-terminal One-Strep-Tag (OST). Such a system allowed the replacement in the genome of SLP-76 by its tagged version and its expression in physiological amounts. Then, by combining high affinity purification and label-free quantitative proteomics, we report here the first description of the SLP76 interactome in resting and activated primary cultured mast cells derived from mice expressing an OST at the C-terminus of SLP-76 (Slp76OST/OST mice).

INSTRUMENT(S): LTQ Orbitrap Velos

ORGANISM(S): Mus Musculus (mouse)

SUBMITTER: Hesse Anne-Marie  

PROVIDER: PXD000052 | Pride | 2013-08-28

REPOSITORIES: Pride

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Publications

Proteomic analysis of the SH2 domain-containing leukocyte protein of 76 kDa (SLP76) interactome in resting and activated primary mast cells [corrected].

Bounab Yacine Y   Hesse Anne-Marie- AM   Iannascoli Bruno B   Grieco Luca L   Couté Yohann Y   Niarakis Anna A   Roncagalli Romain R   Lie Eunkyung E   Lam Kong-Peng KP   Demangel Caroline C   Thieffry Denis D   Garin Jérôme J   Malissen Bernard B   Daëron Marc M  

Molecular & cellular proteomics : MCP 20130702 10


We report the first proteomic analysis of the SLP76 interactome in resting and activated primary mouse mast cells. This was made possible by a novel genetic approach used for the first time here. It consists in generating knock-in mice that express signaling molecules bearing a C-terminal tag that has a high affinity for a streptavidin analog. Tagged molecules can be used as molecular baits to affinity-purify the molecular complex in which they are engaged, which can then be studied by mass spec  ...[more]

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