Ontology highlight
ABSTRACT:
INSTRUMENT(S): LTQ Orbitrap Velos
ORGANISM(S): Homo Sapiens (human)
SUBMITTER: Simone Lemeer
PROVIDER: PXD000138 | Pride | 2013-05-05
REPOSITORIES: Pride
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Marx Harald H Lemeer Simone S Schliep Jan Erik JE Matheron Lucrece L Mohammed Shabaz S Cox Jürgen J Mann Matthias M Heck Albert J R AJ Kuster Bernhard B
Nature biotechnology 20130519 6
We present a peptide library and data resource of >100,000 synthetic, unmodified peptides and their phosphorylated counterparts with known sequences and phosphorylation sites. Analysis of the library by mass spectrometry yielded a data set that we used to evaluate the merits of different search engines (Mascot and Andromeda) and fragmentation methods (beam-type collision-induced dissociation (HCD) and electron transfer dissociation (ETD)) for peptide identification. We also compared the sensitiv ...[more]