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Choroid plexus proteome with and without LPS

ABSTRACT: Choroid plexus was isolated and pooled from the 5 mice after the perfusion with bromo phenol blue from 0 h and 6 h LPS treated mice. Proteins were isolated and trypsin digested and labelled with the NHS-porp (0h –LPS and 6h +LPS). Pre-fractionated using RP-HPLC and analysed on the Qexactive.

INSTRUMENT(S): LTQ Orbitrap

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Cerebrospinal Fluid

SUBMITTER: Sriram Balusu

LAB HEAD: Kris Gevaert

PROVIDER: PXD001347 | Pride | 2016-09-27

REPOSITORIES: Pride

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Identification of a novel mechanism of blood-brain communication during peripheral inflammation via choroid plexus-derived extracellular vesicles.

Balusu Sriram S Van Wonterghem Elien E De Rycke Riet R Raemdonck Koen K Stremersch Stephan S Gevaert Kris K Brkic Marjana M Demeestere Delphine D Vanhooren Valerie V Hendrix An A Libert Claude C Vandenbroucke Roosmarijn E RE

EMBO molecular medicine 20161004 10

Here, we identified release of extracellular vesicles (EVs) by the choroid plexus epithelium (CPE) as a new mechanism of blood-brain communication. Systemic inflammation induced an increase in EVs and associated pro-inflammatory miRNAs, including miR-146a and miR-155, in the CSF Interestingly, this was associated with an increase in amount of multivesicular bodies (MVBs) and exosomes per MVB in the CPE cells. Additionally, we could mimic this using LPS-stimulated primary CPE cells and choroid pl ...[more]

PMID: 27596437

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