Proteomic analysis of integrin adhesion complex assembly

ABSTRACT: To explore the dynamics of integrin adhesion complex assembly, isolated adhesion complexes from K562 cells were subjected to mass spectrometry based proteomic analysis. K562 cells were incubated with FN-coated beads for 1, 7 and 30 minutes and protein complexes stabilised for 2 minutes with DTBP crosslinking reagent before adhesion complexes were isolated.

INSTRUMENT(S): LTQ Velos

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Cell Line Cell

SUBMITTER: Jon Humphries  

LAB HEAD: Martin Humphries

PROVIDER: PXD002159 | Pride | 2015-10-14

REPOSITORIES: Pride