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Analysis of skeletal muscle from a tailored pig model of Duchenne muscular dystrophy

ABSTRACT: We recently generated a novel genetically engineered pig model displaying the fundamental biochemical, clinical and pathological hallmarks of human DMD. To get insight into the hierarchy of molecular derangements during progression of muscular dystrophy, we performed a label free proteome analysis of biceps femoris muscle samples from 2-day-old and 3-month-old DMD and WT pigs.

INSTRUMENT(S): LTQ Orbitrap

ORGANISM(S): Sus Scrofa Domesticus (domestic Pig)

TISSUE(S): Biceps Femoris

DISEASE(S): Duchenne Muscular Dystrophy

SUBMITTER: Thomas Fröhlich

LAB HEAD: Thomas Fröhlich

PROVIDER: PXD002918 | Pride | 2016-09-27

REPOSITORIES: Pride

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Progressive muscle proteome changes in a clinically relevant pig model of Duchenne muscular dystrophy.

Fröhlich Thomas T Kemter Elisabeth E Flenkenthaler Florian F Klymiuk Nikolai N Otte Kathrin A KA Blutke Andreas A Krause Sabine S Walter Maggie C MC Wanke Rüdiger R Wolf Eckhard E Arnold Georg J GJ

Scientific reports 20160916

Duchenne muscular dystrophy (DMD) is caused by genetic deficiency of dystrophin and characterized by massive structural and functional changes of skeletal muscle tissue, leading to terminal muscle failure. We recently generated a novel genetically engineered pig model reflecting pathological hallmarks of human DMD better than the widely used mdx mouse. To get insight into the hierarchy of molecular derangements during DMD progression, we performed a proteome analysis of biceps femoris muscle sam ...[more]

PMID: 27634466

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