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MS imaging of tryptic peptides in mouse brain measured at 50 µm pixel size

ABSTRACT: A coronal mouse brain section that was measured at 50µm pixel size revealed detailed histological structures such as the ependyma (consisting of one to two cell layers, which was confirmed by HE staining).

INSTRUMENT(S): Q Exactive

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Brain

SUBMITTER: Andreas Roempp

LAB HEAD: Andreas Römpp

PROVIDER: PXD003172 | Pride | 2018-08-08

REPOSITORIES: Pride

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Approaching cellular resolution and reliable identification in mass spectrometry imaging of tryptic peptides.

Huber Katharina K Khamehgir-Silz Pegah P Schramm Thorsten T Gorshkov Vladimir V Spengler Bernhard B Römpp Andreas A

Analytical and bioanalytical chemistry 20180801 23

On-tissue digestion has become the preferred method to identify proteins in mass spectrometry (MS) imaging. In this study, we report advances in data acquisition and protein identification for MS imaging after on-tissue digestion. Tryptic peptides in a coronal mouse brain section were measured at 50 μm pixel size and revealed detailed histological structures, e.g., the ependyma (consisting of one to two cell layers), which was confirmed by H&E staining. This demonstrates that MS imaging of trypt ...[more]

PMID: 30066193

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