Proteomics

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Shared and specific targets of Arabidopsis immune MAPKs


ABSTRACT: In Arabidopsis, mitogen-activated protein kinases MPK3, MPK4 and MPK6 constitute essential relays for a variety of functions including cell division, development and innate immunity. While some substrates of MPK3, MPK4 and MPK6 have been identified, the picture is still far from complete. To identify substrates of these MAPKs in cell division, growth and development we compared the phosphoproteomes of wild-type and mpk3, mpk4 and mpk6. To study the function of these MAPKs in innate immunity, we analyzed their phosphoproteomes following activation by a microbe-associated molecular pattern (MAMP). Partially overlapping substrates were retrieved for all three MAPKs, showing target specificity to one, two or all three MAPKs in different biological processes. More precisely, our results illustrate the fact that the entity to be defined as a specific or a shared substrate for MAPKs is not a phosphoprotein but a particular (S/T)P phosphorylation site in a given protein. As a whole, 152 peptides were identified to be differentially phosphorylated in response to MAMP treatment and/or when compared between genotypes and 70 of them could be classified as putative MAPK targets. Biochemical analysis of a number of putative MAPK substrates by phosphorylation and interaction assays confirmed the global phosphoproteome approach. Our study finally expands the set of MAPK substrates to involve other protein kinases, including calcium-dependent (CDPK) and sugar non-fermenting (SnRK) protein kinases.

INSTRUMENT(S): LTQ Orbitrap

ORGANISM(S): Arabidopsis Thaliana (mouse-ear Cress)

SUBMITTER: Delphine PFLIEGER  

LAB HEAD: Heribert Hirt

PROVIDER: PXD006807 | Pride | 2017-12-06

REPOSITORIES: Pride

Dataset's files

Source:
Action DRS
CY_01_3_F_23_Bbis.raw Raw
CY_01_3_F_23_E1.raw Raw
CY_01_3_F_23_E2.raw Raw
CY_01_3_F_S4_B_2.raw Raw
CY_01_3_F_S4_E1.raw Raw
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Publications

Quantitative Phosphoproteomic Analysis Reveals Shared and Specific Targets of <i>Arabidopsis</i> Mitogen-Activated Protein Kinases (MAPKs) MPK3, MPK4, and MPK6.

Rayapuram Naganand N   Bigeard Jean J   Alhoraibi Hanna H   Bonhomme Ludovic L   Hesse Anne-Marie AM   Vinh Joëlle J   Hirt Heribert H   Pflieger Delphine D  

Molecular & cellular proteomics : MCP 20171122 1


In Arabidopsis, mitogen-activated protein kinases MPK3, MPK4, and MPK6 constitute essential relays for a variety of functions including cell division, development and innate immunity. Although some substrates of MPK3, MPK4 and MPK6 have been identified, the picture is still far from complete. To identify substrates of these MAPKs likely involved in cell division, growth and development we compared the phosphoproteomes of wild-type and <i>mpk3</i>, <i>mpk4</i>, and <i>mpk6.</i> To study the funct  ...[more]

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