Ontology highlight
ABSTRACT:
INSTRUMENT(S): LCQ Classic
ORGANISM(S): Pseudomonas Aeruginosa (strain Ucbpp-pa14)
SUBMITTER: Brett Babin
LAB HEAD: David A. Tirrell
PROVIDER: PXD007622 | Pride | 2017-10-24
REPOSITORIES: Pride
mBio 20171024 5
Biofilm infections exhibit high tolerance against antibiotic treatment. The study of biofilms is complicated by phenotypic heterogeneity; biofilm subpopulations differ in their metabolic activities and their responses to antibiotics. Here, we describe the use of the bio-orthogonal noncanonical amino acid tagging (BONCAT) method to enable selective proteomic analysis of a <i>Pseudomonas aeruginosa</i> biofilm subpopulation. Through controlled expression of a mutant methionyl-tRNA synthetase, we t ...[more]