Dataset Information

Hsa-miR-139-5p expression regulates the abundance of proteins related to poor survival cancer in CAL-62 thyroid cancer cell line

ABSTRACT: We studied the impact of hsa-miR-139-5p on the protein output by means of an iTRAQ-based approach. First, we established two CAL-62 isogenic cell lines expressing either the mature hsa-miR-139-5p or a non-targeting control upon a doxycycline inducible promoter (PTRE3G-tGFP, Dharmacon). Total proteins of P-tGFP-hsa-miR139-5p untreated or treated with doxycycline (1ug/ml) for 96 and 120 hours were isolated and labeled with iTRAQ® reagent 8-plex. Two independent experiments were performed.

INSTRUMENT(S): impact

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Cell Culture

DISEASE(S): Thyroid Cancer

SUBMITTER: Eduardo Zarzuela

LAB HEAD: Mercedes Robledo

PROVIDER: PXD011901 | Pride | 2019-08-13

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	20171031_EZ_Cris_iTRAQ_E01_F10_2-C3_01_11773.d.zip	Other
	20171031_EZ_Cris_iTRAQ_E01_F11_2-C4_01_11774.d.zip	Other
	20171031_EZ_Cris_iTRAQ_E01_F12_2-C5_01_11775.d.zip	Other
	20171031_EZ_Cris_iTRAQ_E01_F13_2-C6_01_11776.d.zip	Other
	20171031_EZ_Cris_iTRAQ_E01_F1_2-B2_01_11760.d.zip	Other

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Publications

Hsa-miR-139-5p is a prognostic thyroid cancer marker involved in HNRNPF-mediated alternative splicing.

Montero-Conde Cristina C Graña-Castro Osvaldo O Martín-Serrano Guillermo G Martínez-Montes Ángel M ÁM Zarzuela Eduardo E Muñoz Javier J Torres-Perez Rafael R Pita Guillermo G Cordero-Barreal Alfonso A Leandro-García Luis J LJ Letón Rocío R López de Silanes Isabel I Guadalix Sonsoles S Pérez-Barrios Andrés A Hawkins Federico F Guerrero-Álvarez Almudena A Álvarez-Escolá Cristina C Regojo-Zapata Rita M RM Calsina Bruna B Remacha Laura L Roldán-Romero Juan M JM Santos María M Lanillos Javier J Jordá Mireia M Riesco-Eizaguirre Garcilaso G Zafon Carles C González-Neira Anna A Blasco Maria A MA Al-Shahrour Fátima F Rodríguez-Antona Cristina C Cascón Alberto A Robledo Mercedes M

International journal of cancer 20190828 2

It is critical to identify biomarkers and functional networks associated with aggressive thyroid cancer to anticipate disease progression and facilitate personalized patient management. We performed miRNome sequencing of 46 thyroid tumors enriched with advanced disease patients with a median follow-up of 96 months. MiRNome profiles correlated with tumor-specific histopathological and molecular features, such as stromal cell infiltration and tumor driver mutation. Differential expression analysis ...[more]

PMID: 31403184

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