Proteomics

Dataset Information

0

Determination of GPI structure of human CD59 in HEK293 cells


ABSTRACT: To determine GPI structure of human CD59 in wild-type,B3GALT4-KO, PIGZ-B3GALT4-DKO HEK293 cells.

INSTRUMENT(S): LTQ Orbitrap Velos

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Permanent Cell Line Cell, Epithelial Cell

SUBMITTER: Yicheng Wang  

LAB HEAD: Taroh Kinoshita

PROVIDER: PXD014226 | Pride | 2020-02-17

REPOSITORIES: Pride

Dataset's files

Source:
Action DRS
170706_KO.dat Other
170706_KO.mgf Mgf
170706_KO.raw Raw
170706_WT.dat Other
170706_WT.mgf Mgf
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Publications

Cross-talks of glycosylphosphatidylinositol biosynthesis with glycosphingolipid biosynthesis and ER-associated degradation.

Wang Yicheng Y   Maeda Yusuke Y   Liu Yi-Shi YS   Takada Yoko Y   Ninomiya Akinori A   Hirata Tetsuya T   Fujita Morihisa M   Murakami Yoshiko Y   Kinoshita Taroh T  

Nature communications 20200213 1


Glycosylphosphatidylinositol (GPI)-anchored proteins and glycosphingolipids interact with each other in the mammalian plasma membranes, forming dynamic microdomains. How their interaction starts in the cells has been unclear. Here, based on a genome-wide CRISPR-Cas9 genetic screen for genes required for GPI side-chain modification by galactose in the Golgi apparatus, we report that β1,3-galactosyltransferase 4 (B3GALT4), the previously characterized GM1 ganglioside synthase, additionally functio  ...[more]

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