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Cathepsin B and Cathepsin Z tumor secretome of murine breast cancers

ABSTRACT: MS-based proteomics study of the cancer secretome and tumor interstitial fluid of tumor cell/macrophage co-cultures and breast tumors in cells and mice lacking both cathepsin B and cathepsin Z

INSTRUMENT(S): QSTAR, Q Exactive

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Cell Suspension Culture

DISEASE(S): Breast Cancer

SUBMITTER: Alejandro Gomez-Auli

LAB HEAD: Thomas Reinheckel

PROVIDER: PXD015209 | Pride | 2021-09-08

REPOSITORIES: Pride

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The secreted inhibitor of invasive cell growth CREG1 is negatively regulated by cathepsin proteases.

Gomez-Auli Alejandro A Hillebrand Larissa Elisabeth LE Christen Daniel D Günther Sira Carolin SC Biniossek Martin Lothar ML Peters Christoph C Schilling Oliver O Reinheckel Thomas T

Cellular and molecular life sciences : CMLS 20200508 2

Previous clinical and experimental evidence strongly supports a breast cancer-promoting function of the lysosomal protease cathepsin B. However, the cathepsin B-dependent molecular pathways are not completely understood. Here, we studied the cathepsin-mediated secretome changes in the context of the MMTV-PyMT breast cancer mouse model. Employing the cell-conditioned media from tumor-macrophage co-cultures, as well as tumor interstitial fluid obtained by a novel strategy from PyMT mice with diffe ...[more]

PMID: 32385587

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