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Proteomics of murine melanoma tumor cells upon sphingosine kinase-1 inhibition

ABSTRACT: In order to assess the impact of sphingosine kinase-1 (SK1) expression on melanoma growth, stable SK1 knockdown cells were generated by shRNA, using Yumm 1.7 cells derived from spontaneous murine melanoma driven by Braf activation as well as Pten and Cdkn2a inactivation. Two cell lines silenced for SK1 (shSK1(1) and shSK1(2)) were obtained.

INSTRUMENT(S): LTQ Orbitrap Velos

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Melanocyte, Cell Culture

SUBMITTER: Marlene Marcellin

LAB HEAD: Odile Schiltz

PROVIDER: PXD016535 | Pride | 2019-12-17

REPOSITORIES: Pride

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