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NIMA-related kinase 9–mediated phosphorylation of the microtubule-associated LC3B protein at Thr-50 suppresses selective autophagy of p62/sequestosome 1

ABSTRACT: We found that LC3B is phosphorylated in vitro by STK3, STK4, NEK9, and PKCζ. To identify phosphorylation sites following in vitro phosphorylation, GST-LC3B was overexpressed in E. coli, purified using glutathione-Sepharose 4 Fast Flow beads and incubated in kinase assays with recombinant kinases or FLAG-tagged kinases immunopurified from transiently transfected HEK293 cells

INSTRUMENT(S): Orbitrap Fusion Lumos

ORGANISM(S): Homo Sapiens (human)

SUBMITTER: Jack-Ansgar Bruun

LAB HEAD: Terje Johansen

PROVIDER: PXD016681 | Pride | 2020-01-23

REPOSITORIES: Pride

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NIMA-related kinase 9-mediated phosphorylation of the microtubule-associated LC3B protein at Thr-50 suppresses selective autophagy of p62/sequestosome 1.

Shrestha Birendra Kumar BK Skytte Rasmussen Mads M Abudu Yakubu Princely YP Bruun Jack-Ansgar JA Larsen Kenneth Bowitz KB Alemu Endalkachew A EA Sjøttem Eva E Lamark Trond T Johansen Terje T

The Journal of biological chemistry 20191219 5

Human ATG8 family proteins (ATG8s) are active in all steps of the macroautophagy pathway, and their lipidation is essential for autophagosome formation. Lipidated ATG8s anchored to the outer surface of the phagophore serve as scaffolds for binding of other core autophagy proteins and various effector proteins involved in trafficking or fusion events, whereas those at the inner surface are needed for assembly of selective autophagy substrates. Their scaffolding role depends on specific interactio ...[more]

PMID: 31857374

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