Ontology highlight
ABSTRACT:
INSTRUMENT(S): LTQ Orbitrap Velos
ORGANISM(S): Mus Musculus (mouse)
TISSUE(S): T Cell
SUBMITTER: Mahmoudreza Rafiee
LAB HEAD: Jeroen Krijgsveld
PROVIDER: PXD018764 | Pride | 2020-05-26
REPOSITORIES: Pride
Delacher Michael M Barra Melanie M MM Herzig Yonatan Y Eichelbaum Katrin K Rafiee Mahmoud-Reza MR Richards David M DM Träger Ulrike U Hofer Ann-Cathrin AC Kazakov Alexander A Braband Kathrin L KL Gonzalez Marina M Wöhrl Lukas L Schambeck Kathrin K Imbusch Charles D CD Abramson Jakub J Krijgsveld Jeroen J Feuerer Markus M
iScience 20200504 5
Regulatory T cells are important regulators of the immune system and have versatile functions for the homeostasis and repair of tissues. They express the forkhead box transcription factor Foxp3 as a lineage-defining protein. Negative regulators of Foxp3 expression are not well understood. Here, we generated double-stranded DNA probes complementary to the Foxp3 promoter sequence and performed a pull-down with nuclear protein in vitro, followed by elution of bound proteins and quantitative mass sp ...[more]