Proteomics

Dataset Information

0

HDX-MS of TRAPPII reveals a conserved Rab binding interface

ABSTRACT: We used hydrogen deuterium exchange mass spectrometry (HDX-MS) to define the interface of TRAPPII with three Rab GTPases (Rab1, Rab11, Rab43). These experiments were performed in the presence of EDTA to generate a nucleotide free stabilised Rab-GEF complex. Significant decreases in deuterium incorporation (defined as >4%, >0.4Da, two tailed T-test p value <0.01) were identified in the TRAPPC2L, TRAPPC4, and TRAPPC5 in the presence of Rab GTPases with no other changes in other TRAPP subunits. Here we show that all three Rab GTPases bind at a conserved interface of TRAPPII.

INSTRUMENT(S): impact HD

ORGANISM(S): Homo Sapiens (human)

SUBMITTER: John Burke  

LAB HEAD: John E Burke

PROVIDER: PXD020890 | Pride | 2020-12-11

REPOSITORIES: Pride

Json Xml
altmetric image

Publications

The substrate specificity of the human TRAPPII complex's Rab-guanine nucleotide exchange factor activity.

Jenkins Meredith L ML   Harris Noah J NJ   Dalwadi Udit U   Fleming Kaelin D KD   Ziemianowicz Daniel S DS   Rafiei Atefeh A   Martin Emily M EM   Schriemer David C DC   Yip Calvin K CK   Burke John E JE  

Communications biology 20201204 1

The TRAnsport Protein Particle (TRAPP) complexes act as Guanine nucleotide exchange factors (GEFs) for Rab GTPases, which are master regulators of membrane trafficking in eukaryotic cells. In metazoans, there are two large multi-protein TRAPP complexes: TRAPPII and TRAPPIII, with the TRAPPII complex able to activate both Rab1 and Rab11. Here we present detailed biochemical characterisation of Rab-GEF specificity of the human TRAPPII complex, and molecular insight into Rab binding. GEF assays of  ...[more]

Similar Datasets

Proteomics Detailing the Structural and Dynamic Interactions Between WDR44 and Rab11
2023-03-11 | PXD035741 | Pride
Proteomics Study of the TRAPP complexes by HDX-MS
2021-09-22 | PXD025928 | Pride
Proteomics Detailing the Structural and Dynamic Interactions Between WDR44 and Rab11
2023-02-22 | PXD032837 | Pride
Proteomics Characterization of m-lipin using HDX-MS
2021-06-29 | PXD022172 | Pride
Proteomics Dynamics of allosteric regulation of the phospholipase C-gamma isoform
2022-06-14 | PXD030492 | Pride
Proteomics HDX-MS analysis of PLIN3 membrane binding
2023-04-14 | PXD025717 | Pride
Proteomics Molecular basis for differential activation of p101 and p84 complexes of PI3Kg by Ras and GPCRs
2023-03-09 | PXD035723 | Pride
Proteomics Structure of the phosphoinositide 3-kinase p110-p101 complex reveals molecular mechanism of GPCR activation
2021-09-22 | PXD025209 | Pride
Proteomics A cryo-EM structure of metazoan TRAPPIII, the multisubunit complex that activates the GTPase Rab1
2021-06-03 | PXD025064 | Pride
Proteomics HDX-MS Analysis of the protein ALT51
2023-02-22 | PXD034850 | Pride