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Mouse liver proteome fractionation using leptin-based cartridges.

ABSTRACT: This study is aimed at the evaluation of leptin-based cartridges fractionation for glycoprotein enrichment of mouse liver protein shotgun proteomic profile.

INSTRUMENT(S): Orbitrap Fusion

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Liver

SUBMITTER: Giangiacomo Beretta

LAB HEAD: Giangiacomo Beretta

PROVIDER: PXD025097 | Pride | 2021-07-30

REPOSITORIES: Pride

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DDASSQ: An open-source, multiple peptide sequencing strategy for label free quantification based on an OpenMS pipeline in the KNIME analytics platform.

Svecla Monika M Garrone Giulia G Faré Fiorenza F Aletti Giacomo G Norata Giuseppe Danilo GD Beretta Giangiacomo G

Proteomics 20210801 16

In this study we investigated the performance of a computational pipeline for protein identification and label free quantification (LFQ) of LC-MS/MS data sets from experimental animal tissue samples, as well as the impact of its specific peptide search combinatorial approach. The full pipeline workflow was composed of peptide search engine adapters based on different identification algorithms, in the frame of the open-source OpenMS software running within the KNIME analytics platform. Two differ ...[more]

PMID: 34312990

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