Proteomics

Dataset Information

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EThcD-sceHCD-MS/MS-based Clinical N-glycoproteomics


ABSTRACT: Site-specific N-glycosylation characterization requires intact N-glycopeptide analysis based on suitable tandem mass spectrometry (MS/MS) method. Electron-transfer/higher-energy collisional dissociation (EThcD), stepped collision energy/higher-energy collisional dissociation (sceHCD), and higher-energy collision dissociation-product-dependent electron-transfer dissociation (HCD-pd-ETD) have emerged as valuable approaches for clinical glycoproteomics. However, each of them incurs some compromise, necessitating the performance comparisons when applied to the analysis of complex clinical samples (e.g., plasma, urine, cells, and tissue). Herein, we developed a hybrid mass spectrometry fragmentation method, EThcD-sceHCD, by combining EThcD and sceHCD. Moreover, we compared the performance of this method with those previous approaches (EThcD, sceHCD, HCD-pd-ETD, and sceHCD-pd-ETD) in the intact N-glycopeptide analysis, and determined its applicability for clinical N-glycoproteomic study.

INSTRUMENT(S): Orbitrap Fusion Lumos

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Blood Cell, Blood Plasma

SUBMITTER: Yong Zhang  

LAB HEAD: Yong Zhang

PROVIDER: PXD030288 | Pride | 2022-04-04

REPOSITORIES: Pride

Dataset's files

Source:
Action DRS
20210911_zy_cell_EThcD_1.raw Raw
20210911_zy_cell_EThcD_2.raw Raw
20210911_zy_cell_EThcD_3.raw Raw
20210911_zy_cell_HCD_pd_ETD_1.raw Raw
20210911_zy_cell_HCD_pd_ETD_2.raw Raw
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Publications

Comparative N-Glycoproteomics Analysis of Clinical Samples Via Different Mass Spectrometry Dissociation Methods.

Zeng Wenjuan W   Zheng Shanshan S   Su Tao T   Cheng Jiahan J   Mao Yonghong Y   Zhong Yi Y   Liu Yueqiu Y   Chen Jianhai J   Zhao Wanjun W   Lin Tianhai T   Liu Fang F   Li Guisen G   Yang Hao H   Zhang Yong Y  

Frontiers in chemistry 20220224


Site-specific N-glycosylation characterization requires intact N-glycopeptide analysis based on suitable tandem mass spectrometry (MS/MS) method. Electron-transfer/higher-energy collisional dissociation (EThcD), stepped collision energy/higher-energy collisional dissociation (sceHCD), higher-energy collisional dissociation-product-dependent electron-transfer dissociation (HCD-pd-ETD), and a hybrid mass spectrometry fragmentation method EThcD-sceHCD have emerged as valuable approaches for glycopr  ...[more]

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