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Hypothesis-generating Proteome Perturbation to Identify Molecular Modes of Drug Action: NEU-4438 and Acoziborole in the African Trypanosome

ABSTRACT: Aim of the project was to investigate drug induced proteomic changes in T. brucei within 6 h of treatment with NEU-4438 or SCYX-7158

INSTRUMENT(S): Orbitrap Fusion

ORGANISM(S): Trypanosoma Brucei

TISSUE(S): Cell Culture

DISEASE(S): Trypanosomiasis

SUBMITTER: Amrita Sharma

LAB HEAD: Amrita Sharma

PROVIDER: PXD036393 | Pride | 2023-03-11

REPOSITORIES: Pride

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Hypothesis-generating proteome perturbation to identify NEU-4438 and acoziborole modes of action in the African Trypanosome.

Sharma Amrita A Cipriano Michael M Ferrins Lori L Hajduk Stephen L SL Mensa-Wilmot Kojo K

iScience 20221007 11

NEU-4438 is a lead for the development of drugs against <i>Trypanosoma brucei</i>, which causes human African trypanosomiasis. Optimized with phenotypic screening, targets of NEU-4438 are unknown. Herein, we present a cell perturbome workflow that compares NEU-4438's molecular modes of action to those of SCYX-7158 (acoziborole). Following a 6 h perturbation of trypanosomes, NEU-4438 and acoziborole reduced steady-state amounts of 68 and 92 unique proteins, respectively. After analysis of proteom ...[more]

PMID: 36304107

Publication: 1/2

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