Proteomics

Dataset Information

0

Proteomic comparison of human milk, cow's milk and infant formula extracellular vesicles


ABSTRACT: Milk and milk products such as infant formula (IF) play a fundamental role in serving the nutritional needs of the developing infant. Extracellular vesicles (EVs) in human (HM) and cow’s milk (CM) contain molecular cargo such as proteins and micro(mi)RNA that serve as functional messengers between cells and may be of importance to infant health. Here, we have developed a pipeline using advanced proteomics and transcriptomics to enable cross-species comparison of milk and IF EVs. EVs from HM, CM and IF were subjected to data-independent acquisition mass spectrometry and RNA-seq. Differentially abundant proteins (143) and miRNAs (514) (false discovery rate < 0.01) were identified in HM and CM EVs, and CM EV proteins and miRNAs were conserved in IF EVs (~20-90%). We foresee this work to be used in large scale studies to determine biologically relevant species-specific differences in milk EVs that could be leveraged to improve IF products.

INSTRUMENT(S): TripleTOF 5600

ORGANISM(S): Homo Sapiens (human) Bos Taurus (bovine)

TISSUE(S): Extracellular Vesicle, Milk

DISEASE(S): Disease Free

SUBMITTER: Natalie Turner  

LAB HEAD: Murray D. Mitchell

PROVIDER: PXD039923 | Pride | 2023-08-13

REPOSITORIES: Pride

Dataset's files

Source:
Action DRS
20211222_SWATH_NPT146.wiff Wiff
20211222_SWATH_NPT146.wiff.scan Wiff
20211222_SWATH_NPT147.wiff Wiff
20211222_SWATH_NPT147.wiff.scan Wiff
20211222_SWATH_NPT148.wiff Wiff
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