Ontology highlight
ABSTRACT:
INSTRUMENT(S): Q Exactive HF
ORGANISM(S): Mus Musculus (mouse)
TISSUE(S): Brain
SUBMITTER: Liangliang Sun
LAB HEAD: Liangliang Sun
PROVIDER: PXD042298 | Pride | 2023-12-07
REPOSITORIES: Pride
Wang Qianjie Q Xu Tian T Fang Fei F Wang Qianyi Q Lundquist Peter P Sun Liangliang L
Analytical chemistry 20230818 34
Mass spectrometry (MS)-based top-down characterization of integral membrane proteins (IMPs) is crucial for understanding their functions in biological processes. However, it is technically challenging due to their low solubility in typical MS-compatible buffers. In this work, for the first time, we developed an efficient capillary zone electrophoresis (CZE)-tandem MS (MS/MS) method for the top-down proteomics (TDP) of IMPs enriched from mouse brains. Our technique employs a sample buffer contain ...[more]