Transcriptomics

Dataset Information

3

Comparison of transcriptional response of induced pluripotent stem iPS cell derived and monocyte derived macrophages to bacterial lipopolysaccharide stimulation


ABSTRACT: In the first part of this project, we will differentiate IPS cells from 5 human donors into macrophages, and extract RNA from unstimulated and LPS stimulated macrophages to perform RNA sequencing. We will also extract RNA before and after stimulation in blood- derived macrophages from 5 additional, unrelated healthy samples. In the second part of the project, RNA-seq data will be analysed to compare LPS response of these two macrophage populations. In summary, we will perform 75bp PE RNA-seq on 20 samples (10 pre and post stimulus), on the HiSeq 2500 platform. Samples will be multiplexed at 5 samples / lane, so we will require 4 flow cells in total.This data is part of a pre-publication release. For information on the proper use of pre-publication data shared by the Wellcome Trust Sanger Institute (including details of any publication moratoria), please see http://www.sanger.ac.uk/datasharing/

INSTRUMENT(S): Illumina Genome Analyzer IIx

ORGANISM(S): Homo Sapiens

SUBMITTER: The Wellcome Trust Sanger Institute  

PROVIDER: E-ERAD-192 | ArrayExpress | 2016-05-17

SECONDARY ACCESSION(S): EGAS00001000563

REPOSITORIES: ArrayExpress

Dataset's files

Source:
Action DRS
E-ERAD-192.idf.txt Idf
E-ERAD-192.sdrf.txt Txt
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