Genomics

Dataset Information

303

ChIP-seq of mouse mammary cell line to identify BCL11A targets


ABSTRACT: We have recently identified BCL11A as a key regulator of mammary stem (MaSC) and progenitor cells. Deletion of BCL11A in MaSC leads to loss of ability of those cells to engraft a cleared mammary fatpad. This biological function of BCL11A correlates with its overexpression in basal breast cancers which are thought to arise from stem and progenitor subtypes. Basal breast cancer cell lines where BCL11A is knocked down using shRNA failed to develop tumours in xenograft transplantations in mice. Therefore, BCL11A is potentially a novel target for breast cancer treatment. Microarray analysis has identified potential gene targets for BCL11A however, it is not known if these targets are directly regulated by BCL11A at the transcription level. We would like to perform CHIP-SEQ analysis on a mammary cell line using BCL11A antibody under two conditions (normal expression and overexpression of BCL11A).This data is part of a pre-publication release. For information on the proper use of pre-publication data shared by the Wellcome Trust Sanger Institute (including details of any publication moratoria), please see http://www.sanger.ac.uk/datasharing/protocol to be provided

INSTRUMENT(S): Illumina HiSeq 2000

ORGANISM(S): Mus musculus  

SUBMITTER: The Wellcome Trust Sanger Institute  

PROVIDER: E-ERAD-69 | ArrayExpress | 2013-01-28

SECONDARY ACCESSION(S): ERP001376

REPOSITORIES: ArrayExpress, ENA

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