Transcriptomics

Dataset Information

6

Transcription profiling of zebrafish adult and larval fin to investigate mechanisms of regeneration


ABSTRACT: Zebrafish have the remarkable ability to regenerate body parts including the heart, spinal cord and fins by a process referred to as epimorphic regeneration. Recent studies have illustrated that similar to adult zebrafish, early life stage-larvae also possess the ability to regenerate the caudal fin. A comparative genomic analysis was used to determine the degree of conservation in gene expression among the regenerating adult caudal fin, adult heart and larval fin. Results indicate that these tissues respond to amputation/injury with strikingly similar genomic responses. Comparative analysis revealed raldh2, a rate-limiting enzyme for the synthesis of Retinoic acid (RA), as one of the highly induced genes across the three regeneration platforms. Experiment Overall Design: The caudal fin of zebrafish larvae at 2days post fertilization were amputated. Caudal fin tissue at 2dpf and regenerating fins were isolated at 1, 2and 3 days post amputation. Three replicates were collected at each time point. 150 fins were pooled to comprise one replicate.

INSTRUMENT(S): 418 [Affymetrix]

ORGANISM(S): Danio rerio  

SUBMITTER: Robert L. Tanguay  

PROVIDER: E-GEOD-10188 | ArrayExpress | 2008-04-07

SECONDARY ACCESSION(S): GSE10188PRJNA108295

REPOSITORIES: GEO, ArrayExpress

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