Transcriptomics

Dataset Information

0

RBP2 location analysis


ABSTRACT: RBP2 (JARID1A/RBBP2) enrichment at proximal promoter regions in differentiating promonocytic U937 cells and in the osteosarcoma SAOS-2 cells Keywords: differentiation treatment, ChIP-on-chip analysis We sought to determine transcriptional regulation by RBP2 genome-wide by using location analysis. We describe that RBP2 target genes are separated into two functionally distinct classes: differentiation independent and differentiation dependent genes.Three condition experiment, U937 cells treated with 100 nM 12-O-tetradeconoyl-phorbol 13-acetate (TPA) (Sigma) for two different time points (27 hrs and 96 hrs) and untreated (0 hrs time point or no TPA treatment). In addition, processed data for the unrelated SAOS-2 cells are included. Biological replicates: threechromatin immunoprecipitations (ChIP) performed independently in parallel

ORGANISM(S): Homo sapiens  

SUBMITTER: William G Kaelin Jr   Elizaveta V Benevolenskaya 

PROVIDER: E-GEOD-10636 | ArrayExpress | 2010-06-20

SECONDARY ACCESSION(S): GSE10636PRJNA107707

REPOSITORIES: GEO, ArrayExpress

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Publications

Genome-wide analysis of the H3K4 histone demethylase RBP2 reveals a transcriptional program controlling differentiation.

Lopez-Bigas Nuria N   Kisiel Tomasz A TA   Dewaal Dannielle C DC   Holmes Katie B KB   Volkert Tom L TL   Gupta Sumeet S   Love Jennifer J   Murray Heather L HL   Young Richard A RA   Benevolenskaya Elizaveta V EV  

Molecular cell 20080801 4


Retinoblastoma protein (pRB) mediates cell-cycle withdrawal and differentiation by interacting with a variety of proteins. RB-Binding Protein 2 (RBP2) has been shown to be a key effector. We sought to determine transcriptional regulation by RBP2 genome-wide by using location analysis and gene expression profiling experiments. We describe that RBP2 shows high correlation with the presence of H3K4me3 and its target genes are separated into two functionally distinct classes: differentiation-indepen  ...[more]

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