Transcriptomics

Dataset Information

2

Infection of the larval airway epithelium by Erwinia carotovora


ABSTRACT: The unsurpassed simplicity of the fruitfly’s airway epithelium, that is made of homogenous epithelial cells only, favours its use as a model to study general features and response characteristics of airway epithelia in general. All epithelial cells are able to launch an immune response as characterized by the expression of antimicrobial peptide genes. Infection induces a complex change in the expression profile of these epithelial cells. Outstanding are a priming of the immune system and the launch of a survival program, presumably to counteract infection induced apoptotic signals, which comprises the concurrent expression of known longevity genes such as dFoxo, and dThor. In regions of the airway epithelium with strong immune reactions, a complex remodelling of the airways can be observed, which is characterized by metaplasia and presumably also by hyperplasia of the affected epithelial cells. At the transcriptional level, this reorganization of the airway epithelium is mirrored by a recapitulation of genetic programs that are characteristic for early phases of airway development. Taken together, the response characteristics of the fly’s airway epithelium towards infections discloses features that are known from inflammatory diseases of the human lung, thus opening the opportunity to study fundamental aspects of these diseases in the fly. Keywords:infection, Erwinia c., third instar larva, airway epithelium, two-colour microarray Infection of the airway epithelium by the gram-negativ bacteria Erwinia carotovora. For the infection experiments third instar larvae of the GFP-reporter strain YW DD1 were used and only isolated when the whole epithelium of the airway epithelium showed GFP expression. Uninfected larvae were used as controls. In general, four replicates were performed including dye-swaps. A table of significantly-regulated genes from the SAM-output and GeneTraffic-output has been linked as a supplementary file at the foot of this record.

ORGANISM(S): Drosophila melanogaster  

SUBMITTER: Christina Wagner   Thomas Roeder 

PROVIDER: E-GEOD-11444 | ArrayExpress | 2010-05-19

SECONDARY ACCESSION(S): GSE11444PRJNA109117

REPOSITORIES: GEO, ArrayExpress

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Publications

Molecular architecture of the fruit fly's airway epithelial immune system.

Wagner Christina C   Isermann Kerstin K   Fehrenbach Heinz H   Roeder Thomas T  

BMC genomics 20080929


BACKGROUND: Airway epithelial cells not only constitute a physical barrier, but also the first line of defence against airborne pathogens. At the same time, they are constantly exposed to reactive oxygen species. Therefore, airway epithelia cells have to possess a sophisticated innate immune system and a molecular armamentarium to detoxify reactive oxygen species. It has become apparent that deregulation of epithelial innate immunity is a major reason for the development of chronic inflammatory  ...[more]

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