Transcriptomics

Dataset Information

5

Developmental timecourse of C. elegans small RNAs with 5p monophosphates


ABSTRACT: These datasets profile the expression of small RNAs with 5p monophosphates and 3p hydroxyls (including miRNAs, 21U-RNAs) across C. elegans development and in dauer, glp-4 mutant, and mixed-stage wt worms. These datasets were prepared in the David P. Bartel laboratory using T4 RNA ligase Rnl2(1-249). Keywords: miRNA and 21U-RNA discovery and developmental expression profiling CE-devProfile-5pDependent-Illumina 1 flowcell each for Embryo, L1, L2, L3, L4, Adult, Dauer, Glp-4 mutant; 6 flowcells mixed stage

ORGANISM(S): Caenorhabditis elegans  

SUBMITTER: James C Carrington   Shenghua Duan  Daniel A Chaves  Julie M Claycomb  Jessica J Vasale  David P Bartel  Noah Fahlgren  Gary P Schroth  James Graham Ruby  Craig C Mello  Pedro J Batista  Shujun Luo  Darryl Conte  Kristin D Kasschau  Rosaria Chiang  J G Ruby  Weifung Gu 

PROVIDER: E-GEOD-11734 | ArrayExpress | 2008-06-15

SECONDARY ACCESSION(S): GSE11734PRJNA109167

REPOSITORIES: GEO, ArrayExpress

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Publications


In metazoans, Piwi-related Argonaute proteins have been linked to germline maintenance, and to a class of germline-enriched small RNAs termed piRNAs. Here we show that an abundant class of 21 nucleotide small RNAs (21U-RNAs) are expressed in the C. elegans germline, interact with the C. elegans Piwi family member PRG-1, and depend on PRG-1 activity for their accumulation. The PRG-1 protein is expressed throughout development and localizes to nuage-like structures called P granules. Although 21U-  ...[more]

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