Transcriptomics

Dataset Information

7

Interactions of cytokines in peripheral blood cells from Systemic Lupus Erythematosus


ABSTRACT: SLE patients are always with various disease manifestation. Various cytokines are pointed interacting and playing pathological roles in SLE although the etiopathology is still obscure. In this study, we aimed to investigate the effects of cytokine interactions in the immune response of SLE patients. Overexpressed interferon-inducible(IFI) genes were confirmed in peripheral blood from SLE patients. Using network-based analysis on the immune response-related genes, several networks including cytokines such as TNF and IFN-γ, or beta-estradiol(E2), were constructed. TNF-regulated genes were dominant in these networks but in vitro TNF stimulation on PBMCs showed no different responses in the expressions of these genes between SLE and healthy individuals. Co-stimulating experiments by TNF, IFN-γ, and E2 with IFN-α, revealed that TNF has repressive while IFN-γ essentially has synergistic effect with IFN-α on IFI gene expressions in vitro. E2 showed different effects on IFI gene expressions among 3 individuals. Peripheral blood was obtained from patients with SLE (n=11) and healthy women (n=6). Gene expression profile was analyzed using DNA microarray covering 30,000 human genes. Differentially expressed immune response-related genes were selected and analyzed by using Expression Analysis Systemic Explorer (EASE) based on Gene Ontology (GO) followed by network pathway analysis with Ingenuity Pathways Analysis (IPA).

ORGANISM(S): Homo sapiens  

SUBMITTER: HooiMing Lee   Norihiro Nishimoto  Yasuo Adachi  Kenichi Matsubara  Chieko Aoki  Toru Mima  Hidehiko Sugino  Naoko Yoshio-Hoshino 

PROVIDER: E-GEOD-12374 | ArrayExpress | 2009-05-31

SECONDARY ACCESSION(S): GSE12374PRJNA113211

REPOSITORIES: GEO, ArrayExpress

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Publications

Interactions among type I and type II interferon, tumor necrosis factor, and beta-estradiol in the regulation of immune response-related gene expressions in systemic lupus erythematosus.

Lee Hooi-Ming HM   Mima Toru T   Sugino Hidehiko H   Aoki Chieko C   Adachi Yasuo Y   Yoshio-Hoshino Naoko N   Matsubara Kenichi K   Nishimoto Norihiro N  

Arthritis research & therapy 20090103 1


INTRODUCTION: Systemic lupus erythematosus (SLE) is a prototypical autoimmune disease characterized by various clinical manifestations. Several cytokines interact and play pathological roles in SLE, although the etiopathology is still obscure. In the present study we investigated the network of immune response-related molecules expressed in the peripheral blood of SLE patients, and the effects of cytokine interactions on the regulation of these molecules. METHODS: Gene expression profiles of per  ...[more]

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