Dataset Information


Genome-wide Analysis of Histone Methylations in Memory CD8+ T Cells

ABSTRACT: Histone methylations play a major role in regulating the chromatin state and gene expression, yet little is known about their involvement in differential gene expression and function of memory CD8 T cells. Here, we report a genome-wide analysis of two histone H3 methylations (H3K4me3 and H3K27me3) and gene expression in naïve, central (TCM) and effector (TEM) memory CD8 T cells. Analysis of 16,314 annotated genes in CD8 T cell subsets revealed that gene expression were positively correlated with the levels of H3K4me3 and negatively correlated with the levels of H3K27me3 in these gene loci. The correlation between differential H3K4me3 orH3K27me3 levels with gene expressions in memory CD8 T cells displayed four distinct modes: repressive, active, poised, and bivalent, reflecting their complex regulation and different function of these genes. Furthermore, accessible chromatin states of different gene loci were preferentially influenced by different histone modifications as demonstrated here high levels of H3K9ac found in active gene loci without high levels of H3K4me3. These findings reveal a histone methylation based complex regulation of differential gene expression in memory CD8 T cells. Thus, change of chromatin structure mediated by histone methylation may serve a fundamental basis for the rapid transcriptional response of memory CD8 T cells. genome-wide analysis of two histone H3 methylations (H3K4me3 and H3K27me3) in naïve, central (TCM) and effector (TEM) memory CD8 T cells.


ORGANISM(S): Homo sapiens  

SUBMITTER: Chongzhi Zang   Weiqun Peng  Robert Wersto  William Wood  Zhibin Wang  Tae-Young Roh  Brad Lhotsky  Kevin Becker  Kairong Cui  Keji Zhao  Dustin E Schones  Nan-ping Weng  Yasuto Araki 

PROVIDER: E-GEOD-12616 | ArrayExpress | 2009-06-12



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Genome-wide analysis of histone methylation reveals chromatin state-based regulation of gene transcription and function of memory CD8+ T cells.

Araki Yasuto Y   Wang Zhibin Z   Zang Chongzhi C   Wood William H WH   Schones Dustin D   Cui Kairong K   Roh Tae-Young TY   Lhotsky Brad B   Wersto Robert P RP   Peng Weiqun W   Becker Kevin G KG   Zhao Keji K   Weng Nan-ping NP  

Immunity 20090611 6

Memory lymphocytes are characterized by their ability to exhibit a rapid response to the recall antigen, in which differential transcription is important, yet the underlying mechanism is not understood. We report here a genome-wide analysis of histone methylation on two histone H3 lysine residues (H3K4me3 and H3K27me3) and gene expression profiles in naive and memory CD8(+) T cells. We found that specific correlation exists between gene expression and the amounts of H3K4me3 (positive correlation  ...[more]

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